Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10103 and 10104 are separated by 1303 nucleotides10104 and 10105 are separated by 275 nucleotides10105 and 10106 are separated by 152 nucleotides10106 and 10107 are separated by 403 nucleotides mRNA_1735: 10103 - K03235 EF3, TEF3 elongation factor 3, at 73,051 to 76,843 10103 mRNA_1736: 10104 - K01593 DDC aromatic-L-amino-acid decarboxylase, at 78,147 to 80,608 10104 mRNA_1737: 10105 - KOG1021 Acetylglucosaminyltransferase EXT1/exostosin 1, at 80,884 to 82,688 10105 mRNA_1738: 10106 - Hypothetical Protein, at 82,841 to 84,052 10106 mRNA_1739: 10107 - Hypothetical Protein, at 84,456 to 87,207 10107
Group Condition mRNA_1735 mRNA_1736 mRNA_1737 mRNA_1738 mRNA_1739
10103 10104 10105 10106 10107
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -0.3 -0.6 -1.0
growth on ypd YPD N.D. -0.0 +0.1 -0.4 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 -0.4 -0.6 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 -0.3 -0.4 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -0.1 +0.3 -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.1 -0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.3 -1.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 -0.2 -0.3 +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.2 -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 +0.0 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 -0.3 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 +0.2 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.3 -0.1 +0.3
growth on oleic acid YNB Glucose N.D. -0.0 +0.1 +0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.0 -0.2 +0.0
growth on ricinoleic acid YNB Glucose N.D. -0.1 +0.3 +0.3 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 +0.1 +0.2 +0.0
auxotrophy YNB Glucose N.D. +0.1 +0.1 +0.0 +0.1
auxotrophy Dropout Complete N.D. +0.1 +0.3 +0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 +0.4 +0.4 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 +0.1 +0.4 +0.0
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