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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10118 and 10119 are separated by 144 nucleotides
10119 and 10120 are separated by 163 nucleotides
10120 and 10121 are separated by 278 nucleotides
10121 and 10122 are separated by 36 nucleotides
mRNA_1750: 10118 - K16185 RRAGA_B Ras-related GTP-binding protein A/B, at 113,201 to 114,530
10118
mRNA_1751: 10119 - KOG2726 Mitochondrial polypeptide chain release factor, at 114,675 to 115,608
10119
mRNA_1752: 10120 - K15363 FAN1, MTMR15 fanconi-associated nuclease 1, at 115,772 to 119,229
10120
mRNA_1753: 10121 - BLAST ubiquitin ligase SCF complex subunit Cullin [Rhizocto..., at 119,508 to 121,647
10121
mRNA_1754: 10122 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 121,684 to 123,500
10122
Group
Condition
mRNA
_1750
mRNA
_1751
mRNA
_1752
mRNA
_1753
mRNA
_1754
10118
10119
10120
10121
10122
ypd buoyancy
40 hrs High Buoyancy
N.D.
-0.8
-0.0
-0.1
+0.0
auxotrophy
Dropout Complete
N.D.
-0.4
-0.1
-0.2
+0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
-0.3
+0.2
-0.4
+0.1
auxotrophy
YNB Glucose 75mM Arginine
N.D.
-0.5
-0.0
+0.0
+0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
-0.1
-0.0
+0.2
-0.3
growth on ricinoleic acid
YNB Glucose
N.D.
-0.1
-0.0
+0.1
-0.2
ypd buoyancy
40 hrs Median Buoyancy
N.D.
-0.2
+0.1
-0.0
+0.0
growth on oleic acid
YNB Oleic Acid
N.D.
+0.0
+0.1
-0.1
-0.1
auxotrophy
YNB Glucose 75mM Methionine
N.D.
-0.2
-0.0
+0.1
-0.0
ypd buoyancy
40 hrs Low Buoyancy
N.D.
-0.2
+0.1
-0.2
+0.3
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
+0.0
+0.2
+0.0
-0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
+0.1
-0.1
+0.3
-0.3
auxotrophy
YNB Glucose
N.D.
+0.0
+0.0
-0.0
+0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
-0.0
-0.1
+0.2
+0.0
growth on ypd
YPD
N.D.
+0.7
-0.0
-0.1
-0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
+0.5
-0.0
+0.4
-0.4
growth on oleic acid
YNB Glucose
N.D.
+0.4
+0.1
-0.0
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
-0.0
+0.1
+0.2
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
-0.0
+0.3
+0.0
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
+0.6
+0.1
+0.2
-0.2
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
+1.0
+0.2
+0.0
+0.2
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