Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10123 and 10124 are separated by 157 nucleotides10124 and 10125 are separated by 153 nucleotides10125 and 10126 are separated by 706 nucleotides10126 and 10127 are separated by 1682 nucleotides mRNA_1755: 10123 - K00326 E1.6.2.2 cytochrome-b5 reductase, at 123,629 to 124,708 10123 mRNA_1756: 10124 - KOG2821 RNA polymerase II transcription elongation factor Elongin/SIII, subunit elongin A, at 124,866 to 126,194 10124 mRNA_1757: 10125 - KOG1426 FOG RCC1 domain, at 126,348 to 129,051 10125 mRNA_1758: 10126 - KOG2998 Uncharacterized conserved protein, at 129,758 to 131,068 10126 mRNA_1759: 10127 - K13345 PEX12, PAF3 peroxin-12, at 132,751 to 134,282 10127
Group Condition mRNA_1755 mRNA_1756 mRNA_1757 mRNA_1758 mRNA_1759
10123 10124 10125 10126 10127
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.4 +0.1 -1.7 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.3 +0.1 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.5 +0.2 +0.1 +0.7 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.3 -0.1 +0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.1 -0.2 +0.0 N.D.
growth on ypd YPD +0.3 -0.1 +0.3 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 -0.2 -0.0 +0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +1.1 -0.1 -0.3 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 -0.3 -0.3 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.1 -0.1 +0.2 N.D.
auxotrophy Dropout Complete +1.8 +0.2 -0.3 -0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine +1.6 +0.1 -0.1 -0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine +1.6 +0.1 -0.1 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.0 +0.2 +0.4 N.D.
auxotrophy YNB Glucose +1.8 +0.1 -0.1 -0.3 N.D.
growth on oleic acid YNB Glucose +1.4 +0.3 -0.2 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.6 +0.1 +0.2 +0.8 N.D.
growth on oleic acid YNB Oleic Acid +2.2 +0.0 +0.1 -0.1 N.D.
growth on ricinoleic acid YNB Glucose +2.3 -0.2 -0.0 +0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +3.4 -0.3 +0.0 +0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +4.4 -0.0 -0.1 -0.1 N.D.
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