Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10124 and 10125 are separated by 153 nucleotides10125 and 10126 are separated by 706 nucleotides10126 and 10127 are separated by 1682 nucleotides10127 and 10128 are separated by 429 nucleotides mRNA_1756: 10124 - KOG2821 RNA polymerase II transcription elongation factor Elongin/SIII, subunit elongin A, at 124,866 to 126,194 10124 mRNA_1757: 10125 - KOG1426 FOG RCC1 domain, at 126,348 to 129,051 10125 mRNA_1758: 10126 - KOG2998 Uncharacterized conserved protein, at 129,758 to 131,068 10126 mRNA_1759: 10127 - K13345 PEX12, PAF3 peroxin-12, at 132,751 to 134,282 10127 mRNA_1760: 10128 - KOG0940 Ubiquitin protein ligase RSP5/NEDD4, at 134,712 to 138,303 10128
Group Condition mRNA_1756 mRNA_1757 mRNA_1758 mRNA_1759 mRNA_1760
10124 10125 10126 10127 10128
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.1 -1.7 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.1 -0.4 N.D. -0.4
auxotrophy Dropout Complete +0.2 -0.3 -0.6 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 -0.4 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.1 -0.3 N.D. -0.1
auxotrophy YNB Glucose +0.1 -0.1 -0.3 N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.3 +0.5 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.3 -0.1 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.1 +0.4 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.2 +0.0 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.1 -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.0 +0.3 N.D. +0.1
growth on oleic acid YNB Glucose +0.3 -0.2 +0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.0 +0.1 -0.1 N.D. +0.2
growth on ypd YPD -0.1 +0.3 -0.2 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.0 +0.4 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 +0.2 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.2 +0.4 N.D. +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.1 +0.7 N.D. -0.3
growth on ricinoleic acid YNB Glucose -0.2 -0.0 +0.5 N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.2 +0.8 N.D. +0.1
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