Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10125 and 10126 are separated by 706 nucleotides10126 and 10127 are separated by 1682 nucleotides10127 and 10128 are separated by 429 nucleotides10128 and 10129 are separated by 3523 nucleotides mRNA_1757: 10125 - KOG1426 FOG RCC1 domain, at 126,348 to 129,051 10125 mRNA_1758: 10126 - KOG2998 Uncharacterized conserved protein, at 129,758 to 131,068 10126 mRNA_1759: 10127 - K13345 PEX12, PAF3 peroxin-12, at 132,751 to 134,282 10127 mRNA_1760: 10128 - KOG0940 Ubiquitin protein ligase RSP5/NEDD4, at 134,712 to 138,303 10128 mRNA_1761: 10129 - K07189 PPP1R3 protein phosphatase 1 regulatory subunit 3A/B/C/D/E, at 141,827 to 142,144 10129
Group Condition mRNA_1757 mRNA_1758 mRNA_1759 mRNA_1760 mRNA_1761
10125 10126 10127 10128 10129
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.7 N.D. +0.1 -1.6
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.0 N.D. +0.2 -0.8
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.4 N.D. -0.4 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.1 N.D. +0.3 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.7 N.D. -0.3 -1.1
growth on oleic acid YNB Glucose -0.2 +0.0 N.D. +0.1 -0.2
auxotrophy Dropout Complete -0.3 -0.6 N.D. +0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.4 N.D. +0.0 +0.3
auxotrophy YNB Glucose -0.1 -0.3 N.D. -0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.1 N.D. +0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.4 N.D. +0.0 -0.6
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 N.D. -0.1 +0.6
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. +0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.4 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.4 N.D. +0.2 -0.2
growth on ypd YPD +0.3 -0.2 N.D. +0.2 +0.5
growth on ricinoleic acid YNB Glucose -0.0 +0.5 N.D. +0.5 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.3 N.D. +0.1 +1.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.8 N.D. +0.1 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.5 N.D. -0.1 +1.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 N.D. +0.3 +1.8
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