Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10126 and 10127 are separated by 1682 nucleotides10127 and 10128 are separated by 429 nucleotides10128 and 10129 are separated by 3523 nucleotides10129 and 10130 are separated by 572 nucleotides mRNA_1758: 10126 - KOG2998 Uncharacterized conserved protein, at 129,758 to 131,068 10126 mRNA_1759: 10127 - K13345 PEX12, PAF3 peroxin-12, at 132,751 to 134,282 10127 mRNA_1760: 10128 - KOG0940 Ubiquitin protein ligase RSP5/NEDD4, at 134,712 to 138,303 10128 mRNA_1761: 10129 - K07189 PPP1R3 protein phosphatase 1 regulatory subunit 3A/B/C/D/E, at 141,827 to 142,144 10129 mRNA_1762: 10130 - Hypothetical Protein, at 142,717 to 145,078 10130
Group Condition mRNA_1758 mRNA_1759 mRNA_1760 mRNA_1761 mRNA_1762
10126 10127 10128 10129 10130
facs on lipid accumulation Sorted Low BODIPY Gate -1.7 N.D. +0.1 -1.6 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.4 N.D. -0.4 +0.1 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 N.D. -0.3 -1.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. +0.2 -0.8 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. +0.3 -0.5 -0.3
auxotrophy Dropout Complete -0.6 N.D. +0.2 +0.4 -0.3
auxotrophy YNB Glucose -0.3 N.D. -0.0 +0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.3 -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.1 +0.6 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. +0.0 +0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.1 +0.2 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. +0.0 -0.6 +0.2
growth on oleic acid YNB Glucose +0.0 N.D. +0.1 -0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. +0.2 -0.2 -0.3
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.2 -0.0 -0.0
growth on ypd YPD -0.2 N.D. +0.2 +0.5 -0.0
growth on ricinoleic acid YNB Glucose +0.5 N.D. +0.5 +0.4 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.8 N.D. +0.1 +0.8 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. +0.1 +1.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. -0.1 +1.9 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.3 +1.8 +0.1
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