Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10128 and 10129 are separated by 3523 nucleotides10129 and 10130 are separated by 572 nucleotides10130 and 10131 are separated by 364 nucleotides10131 and 10132 are separated by 153 nucleotides mRNA_1760: 10128 - KOG0940 Ubiquitin protein ligase RSP5/NEDD4, at 134,712 to 138,303 10128 mRNA_1761: 10129 - K07189 PPP1R3 protein phosphatase 1 regulatory subunit 3A/B/C/D/E, at 141,827 to 142,144 10129 mRNA_1762: 10130 - Hypothetical Protein, at 142,717 to 145,078 10130 mRNA_1763: 10131 - Hypothetical Protein, at 145,443 to 147,690 10131 mRNA_1764: 10132 - KOG0867 Glutathione S-transferase, at 147,844 to 149,167 10132
Group Condition mRNA_1760 mRNA_1761 mRNA_1762 mRNA_1763 mRNA_1764
10128 10129 10130 10131 10132
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.6 -0.2 -0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -1.1 -0.1 -0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.1 -0.6 -0.4 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.5 -0.3 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.6 +0.2 -0.4 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.2 -0.3 -0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.8 -0.1 +0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 -0.5 +0.0 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.2 -0.1 +0.0 -0.1
growth on oleic acid YNB Oleic Acid +0.2 -0.0 -0.0 -0.1 +0.3
growth on ricinoleic acid YNB Glucose +0.5 +0.4 -0.3 -0.0 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.6 -0.3 +0.0 +0.4
auxotrophy YNB Glucose -0.0 +0.2 -0.2 +0.2 +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.8 -0.2 +0.0 +0.1
growth on oleic acid YNB Glucose +0.1 -0.2 +0.2 +0.1 +0.6
auxotrophy Dropout Complete +0.2 +0.4 -0.3 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +1.2 -0.1 -0.8 +0.6
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.3 +0.1 +0.3 +0.4
growth on ypd YPD +0.2 +0.5 -0.0 +0.7 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +1.9 +0.1 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +1.8 +0.1 -0.4 +0.4
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