Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10132 and 10133 are separated by 206 nucleotides10133 and 10134 are separated by 1763 nucleotides10134 and 10135 are separated by 364 nucleotides10135 and 10136 are separated by 203 nucleotides mRNA_1764: 10132 - KOG0867 Glutathione S-transferase, at 147,844 to 149,167 10132 mRNA_1765: 10133 - HMMPfam-FUN14 family-PF04930, at 149,374 to 150,218 10133 mRNA_1766: 10134 - K03097 CSNK2A casein kinase II subunit alpha, at 151,982 to 154,408 10134 mRNA_1767: 10135 - HMMPfam-F-box-like-PF12937, at 154,773 to 156,142 10135 mRNA_1768: 10136 - K01858 INO1, ISYNA1 myo-inositol-1-phosphate synthase, at 156,346 to 158,351 10136
Group Condition mRNA_1764 mRNA_1765 mRNA_1766 mRNA_1767 mRNA_1768
10132 10133 10134 10135 10136
growth on ypd YPD -0.1 -0.3 -0.1 -0.1 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.5 -0.4 +0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.9 +0.5 -0.2 +0.0
growth on oleic acid YNB Oleic Acid +0.3 +0.1 -0.1 -0.5 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.9 -0.3 +0.7 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.4 +0.1 +0.3 -1.0
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.2 -0.4 +0.3 -0.2
growth on oleic acid YNB Glucose +0.6 -0.2 +0.1 -0.4 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.0 -0.1 +0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.2 -0.3 +0.5 -0.6
auxotrophy Dropout Complete +0.3 -0.1 +0.3 -0.0 -0.7
growth on ricinoleic acid YNB Glucose +0.0 -0.1 -0.1 +0.2 -0.1
auxotrophy YNB Glucose +0.5 -0.4 +0.1 +0.5 -0.4
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.2 +0.3 +0.3 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.0 -0.1 +0.4 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.2 +0.7 +1.0 -0.6
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.4 -0.2 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.3 +0.5 +0.8 -1.1
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.2 +0.5 +0.7 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.5 +0.4 +0.7 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.3 +0.5 +0.6 -0.2
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