Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10139 and 10140 are separated by 945 nucleotides10140 and 10141 are separated by 749 nucleotides10141 and 10142 are separated by 252 nucleotides10142 and 10143 are separated by 285 nucleotides mRNA_1771: 10139 - K14312 NUP155, NUP170, NUP157 nuclear pore complex protein Nup155, at 163,525 to 168,544 10139 mRNA_1772: 10140 - K01480 speB agmatinase, at 169,490 to 170,600 10140 mRNA_1773: 10141 - Hypothetical Protein, at 171,350 to 172,928 10141 mRNA_1774: 10142 - Hypothetical Protein, at 173,181 to 173,906 10142 mRNA_1775: 10143 - KOG1289 Amino acid transporters, at 174,192 to 176,320 10143
Group Condition mRNA_1771 mRNA_1772 mRNA_1773 mRNA_1774 mRNA_1775
10139 10140 10141 10142 10143
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 -0.3 -0.6 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 -0.5 -0.4 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.1 -0.3 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.1 +0.0 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 +0.3 -0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -0.2 -0.2 +0.0
auxotrophy Dropout Complete N.D. +0.0 -0.0 +0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 +0.1 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 -0.1 -0.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 +0.3 -0.3 -0.2
growth on oleic acid YNB Glucose N.D. +0.1 +0.1 -0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 +0.2 -0.1 -0.3
growth on ricinoleic acid YNB Glucose N.D. +0.3 +0.1 +0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 +0.1 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 +0.3 +0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.2 +0.2 -0.1
auxotrophy YNB Glucose N.D. +0.4 +0.2 +0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.5 +0.5 -0.1 -0.3
growth on ypd YPD N.D. +0.1 +0.0 +0.5 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 +0.1 +0.3 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.3 +0.5 +0.1
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