Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10141 and 10142 are separated by 252 nucleotides10142 and 10143 are separated by 285 nucleotides10143 and 10144 are separated by 983 nucleotides10144 and 10145 are separated by 622 nucleotides mRNA_1773: 10141 - Hypothetical Protein, at 171,350 to 172,928 10141 mRNA_1774: 10142 - Hypothetical Protein, at 173,181 to 173,906 10142 mRNA_1775: 10143 - KOG1289 Amino acid transporters, at 174,192 to 176,320 10143 mRNA_1776: 10144 - K01869 LARS, leuS leucyl-tRNA synthetase, at 177,304 to 181,065 10144 mRNA_1777: 10145 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 181,688 to 183,339 10145
Group Condition mRNA_1773 mRNA_1774 mRNA_1775 mRNA_1776 mRNA_1777
10141 10142 10143 10144 10145
auxotrophy Dropout Complete -0.0 +0.1 -0.3 N.D. -0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.4 +0.0 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.6 -0.1 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 -0.3 N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.3 -0.3 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.1 -0.3 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.1 +0.0 -0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose +0.1 +0.0 -0.2 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.2 +0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.3 -0.2 N.D. +0.1
auxotrophy YNB Glucose +0.2 +0.2 -0.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.0 +0.0 N.D. +0.0
growth on oleic acid YNB Glucose +0.1 -0.0 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.3 -0.1 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.2 -0.1 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.2 -0.1 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 +0.1 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.3 -0.1 N.D. -0.0
growth on ypd YPD +0.0 +0.5 +0.1 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.3 +0.4 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.5 +0.1 N.D. +0.3
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