Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10143 and 10144 are separated by 983 nucleotides10144 and 10145 are separated by 622 nucleotides10145 and 10146 are separated by 559 nucleotides10146 and 10147 are separated by 571 nucleotides mRNA_1775: 10143 - KOG1289 Amino acid transporters, at 174,192 to 176,320 10143 mRNA_1776: 10144 - K01869 LARS, leuS leucyl-tRNA synthetase, at 177,304 to 181,065 10144 mRNA_1777: 10145 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 181,688 to 183,339 10145 mRNA_1778: 10146 - HMMPfam-Protein of unknown function (DUF3140)-PF11338, at 183,899 to 184,487 10146 mRNA_1779: 10147 - KOG1336 Monodehydroascorbate/ferredoxin reductase, at 185,059 to 187,419 10147
Group Condition mRNA_1775 mRNA_1776 mRNA_1777 mRNA_1778 mRNA_1779
10143 10144 10145 10146 10147
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.0 -0.2 -1.1
auxotrophy Dropout Complete -0.3 N.D. -0.6 -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.4 -0.2 +0.4
growth on ricinoleic acid YNB Glucose -0.2 N.D. -0.2 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.1 -0.4 -0.2
auxotrophy YNB Glucose -0.3 N.D. -0.3 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. +0.1 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.3 -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.0 +0.1 -0.2
growth on oleic acid YNB Glucose -0.1 N.D. -0.1 -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. -0.1 -0.3 +0.5
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.2 -0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. -0.4 +0.3 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.3 -0.3 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. +0.0 +0.0 +0.2
growth on ypd YPD +0.1 N.D. +0.1 +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. +0.3 -0.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. +0.1 +0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.3 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.0 +0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. +0.1 +0.4 +0.1
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