Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10148 and 10149 are separated by 1370 nucleotides10149 and 10150 are separated by 277 nucleotides10150 and 10151 are separated by 4313 nucleotides10151 and 10152 are separated by 1908 nucleotides mRNA_1780: 10148 - K03097 CSNK2A casein kinase II subunit alpha, at 188,095 to 190,025 10148 mRNA_1781: 10149 - K15109 SLC25A20_29, CACT, CACL, CRC1 solute carrier family 25 (mitochondrial carnitine/acylcarnitine transporter), member 20/29, at 191,396 to 193,155 10149 mRNA_1782: 10150 - Hypothetical Protein, at 193,433 to 193,902 10150 mRNA_1783: 10151 - HMMPfam-Domain of unknown function (DUF4211)-PF13926, at 198,216 to 199,612 10151 mRNA_1784: 10152 - KOG0257 Kynurenine aminotransferase, glutamine transaminase K, at 201,521 to 204,096 10152
Group Condition mRNA_1780 mRNA_1781 mRNA_1782 mRNA_1783 mRNA_1784
10148 10149 10150 10151 10152
auxotrophy Dropout Complete N.D. -0.5 N.D. -0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.5 N.D. +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 N.D. -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 N.D. +0.3 -0.5
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. -0.3 +0.1
auxotrophy YNB Glucose N.D. -0.2 N.D. -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. +0.0 -0.0
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. +0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 N.D. +0.4 -0.1
growth on oleic acid YNB Glucose N.D. -0.2 N.D. +0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 N.D. +0.3 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 N.D. +0.2 +0.2
growth on ypd YPD N.D. -0.2 N.D. -0.1 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.4 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 N.D. +0.1 +0.4
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. +0.4 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.8 N.D. -0.1 +0.6
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