Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10153 and 10154 are separated by 185 nucleotides10154 and 10155 are separated by 414 nucleotides10155 and 10156 are separated by 168 nucleotides10156 and 10157 are separated by 522 nucleotides mRNA_1785: 10153 - K04077 groEL, HSPD1 chaperonin GroEL, at 204,844 to 206,779 10153 mRNA_1786: 10154 - K04078 groES, HSPE1 chaperonin GroES, at 206,965 to 207,485 10154 mRNA_1787: 10155 - HMMPfam-Tctex-1 family-PF03645, at 207,900 to 208,690 10155 mRNA_1788: 10156 - K16368 DGK1 diacylglycerol kinase (CTP), at 208,859 to 210,446 10156 mRNA_1789: 10157 - K10573 UBE2A, UBC2, RAD6A ubiquitin-conjugating enzyme E2 A, at 210,969 to 211,922 10157
Group Condition mRNA_1785 mRNA_1786 mRNA_1787 mRNA_1788 mRNA_1789
10153 10154 10155 10156 10157
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.0 -0.5 N.D.
auxotrophy Dropout Complete N.D. N.D. -0.1 -1.4 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 -1.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.3 -1.0 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.3 -1.3 N.D.
growth on oleic acid YNB Glucose N.D. N.D. -0.2 -0.8 N.D.
growth on ypd YPD N.D. N.D. -0.1 -0.8 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.4 -0.4 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 -0.9 N.D.
auxotrophy YNB Glucose N.D. N.D. +0.2 -0.9 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.4 -0.8 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.0 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.5 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.5 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.8 -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.0 +1.2 N.D.
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