Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10155 and 10156 are separated by 168 nucleotides10156 and 10157 are separated by 522 nucleotides10157 and 10158 are separated by 261 nucleotides10158 and 10159 are separated by 248 nucleotides mRNA_1787: 10155 - HMMPfam-Tctex-1 family-PF03645, at 207,900 to 208,690 10155 mRNA_1788: 10156 - K16368 DGK1 diacylglycerol kinase (CTP), at 208,859 to 210,446 10156 mRNA_1789: 10157 - K10573 UBE2A, UBC2, RAD6A ubiquitin-conjugating enzyme E2 A, at 210,969 to 211,922 10157 mRNA_1790: 10158 - Hypothetical Protein, at 212,184 to 213,881 10158 mRNA_1791: 10159 - Hypothetical Protein, at 214,130 to 214,450 10159
Group Condition mRNA_1787 mRNA_1788 mRNA_1789 mRNA_1790 mRNA_1791
10155 10156 10157 10158 10159
auxotrophy Dropout Complete -0.1 -1.4 N.D. +0.3 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.5 N.D. -0.0 +0.4
growth on oleic acid YNB Glucose -0.2 -0.8 N.D. -0.3 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 -1.4 N.D. +0.6 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -1.0 N.D. +0.3 +0.2
growth on ypd YPD -0.1 -0.8 N.D. +0.4 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 -1.3 N.D. +0.4 -0.0
growth on ricinoleic acid YNB Glucose +0.1 -0.9 N.D. +0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.4 N.D. +0.3 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 N.D. -0.0 +0.1
auxotrophy YNB Glucose +0.2 -0.9 N.D. +0.5 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.4 N.D. +0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.1 N.D. -0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.4 N.D. +0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.0 -0.3 N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.8 N.D. +0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 N.D. +0.0 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +1.2 N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 +0.2 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.0 N.D. +0.1 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 -0.0 N.D. +0.1 +0.2
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