Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10164 and 10165 are separated by 110 nucleotides10165 and 10166 are separated by 513 nucleotides10166 and 10167 are separated by 149 nucleotides10167 and 10168 are separated by 149 nucleotides mRNA_1796: 10164 - K10878 SPO11 meiotic recombination protein SPO11, at 219,619 to 222,270 10164 mRNA_1797: 10165 - KOG2641 Predicted seven transmembrane receptor - rhodopsin family, at 222,381 to 224,884 10165 mRNA_1798: 10166 - KOG0725 Reductases with broad range of substrate specificities, at 225,398 to 226,777 10166 mRNA_1799: 10167 - KOG2489 Transmembrane protein, at 226,927 to 229,515 10167 mRNA_1800: 10168 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 229,665 to 231,047 10168
Group Condition mRNA_1796 mRNA_1797 mRNA_1798 mRNA_1799 mRNA_1800
10164 10165 10166 10167 10168
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 -0.6 -0.3 -0.3 +0.2
auxotrophy Dropout Complete -0.7 -0.3 -0.6 -0.2 +0.0
growth on oleic acid YNB Glucose -1.0 -0.0 +0.0 -0.4 -0.1
growth on oleic acid YNB Oleic Acid -0.5 -0.4 +0.0 -0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.5 -0.1 -0.4 +0.3
growth on ricinoleic acid YNB Glucose -0.7 -0.3 -0.1 -0.2 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.9 -0.1 -0.2 -0.1 +0.1
auxotrophy YNB Glucose -0.4 -0.1 -0.2 -0.2 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.6 -0.2 -0.2 +0.0 +0.3
growth on ypd YPD +0.2 +0.2 -0.4 +0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.3 -0.1 -0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.1 -0.1 -0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.5 -0.3 +0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.3 -0.1 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.9 +0.0 -0.1 -0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 +0.2 -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 +0.1 +0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.4 +0.3 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.1 +0.4 -0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.0 -0.1 -0.1 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 +0.1 +0.1 -0.1 -0.0
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