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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10166 and 10167 are separated by 149 nucleotides
10167 and 10168 are separated by 149 nucleotides
10168 and 10169 are separated by 137 nucleotides
10169 and 10170 are separated by 74 nucleotides
mRNA_1798: 10166 - KOG0725 Reductases with broad range of substrate specificities, at 225,398 to 226,777
10166
mRNA_1799: 10167 - KOG2489 Transmembrane protein, at 226,927 to 229,515
10167
mRNA_1800: 10168 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 229,665 to 231,047
10168
mRNA_1801: 10169 - HMMPfam-Survival motor neuron (SMN) interacting protein 1 (SIP1)-PF04938, at 231,185 to 232,547
10169
mRNA_1802: 10170 - K05546 GANAB alpha 1,3-glucosidase, at 232,622 to 238,014
10170
Group
Condition
mRNA
_1798
mRNA
_1799
mRNA
_1800
mRNA
_1801
mRNA
_1802
10166
10167
10168
10169
10170
auxotrophy
Dropout Complete
-0.6
-0.2
+0.0
N.D.
-0.7
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.3
-0.3
+0.2
N.D.
-0.8
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
-0.4
+0.3
N.D.
-1.1
growth on ricinoleic acid
YNB Glucose
-0.1
-0.2
+0.0
N.D.
-0.9
auxotrophy
YNB Glucose
-0.2
-0.2
+0.1
N.D.
-0.8
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
-0.2
-0.3
N.D.
-0.6
facs on lipid accumulation
Sorted High BODIPY Gate
-0.1
-0.1
-0.0
N.D.
-0.5
growth on oleic acid
YNB Glucose
+0.0
-0.4
-0.1
N.D.
-0.3
auxotrophy
YNB Glucose 75mM Arginine
-0.2
-0.1
+0.1
N.D.
-0.6
growth on oleic acid
YNB Oleic Acid
+0.0
-0.3
-0.2
N.D.
-0.3
growth on ypd
YPD
-0.4
+0.1
-0.2
N.D.
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
-0.1
-0.0
N.D.
-0.4
auxotrophy
YNB Glucose 75mM Methionine
-0.2
+0.0
+0.3
N.D.
-0.6
ypd buoyancy
40 hrs High Buoyancy
-0.1
-0.1
+0.0
N.D.
-0.1
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.1
-0.2
+0.0
N.D.
+0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.1
-0.3
+0.1
N.D.
+0.5
ypd buoyancy
40 hrs Median Buoyancy
+0.2
-0.1
+0.1
N.D.
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.4
-0.2
+0.3
N.D.
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
+0.1
+0.1
+0.1
N.D.
+0.2
ypd buoyancy
40 hrs No Buoyancy Separation
-0.3
+0.3
+0.1
N.D.
+0.8
ypd buoyancy
40 hrs Low Buoyancy
+0.3
-0.1
+0.2
N.D.
+0.7
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