Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10168 and 10169 are separated by 137 nucleotides10169 and 10170 are separated by 74 nucleotides10170 and 10171 are separated by 292 nucleotides10171 and 10172 are separated by 177 nucleotides mRNA_1800: 10168 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 229,665 to 231,047 10168 mRNA_1801: 10169 - HMMPfam-Survival motor neuron (SMN) interacting protein 1 (SIP1)-PF04938, at 231,185 to 232,547 10169 mRNA_1802: 10170 - K05546 GANAB alpha 1,3-glucosidase, at 232,622 to 238,014 10170 mRNA_1803: 10171 - K15205 TFC6 transcription factor C subunit 6, at 238,307 to 241,610 10171 mRNA_1804: 10172 - Hypothetical Protein, at 241,788 to 243,277 10172
Group Condition mRNA_1800 mRNA_1801 mRNA_1802 mRNA_1803 mRNA_1804
10168 10169 10170 10171 10172
auxotrophy Dropout Complete +0.0 N.D. -0.7 N.D. -1.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -1.1 N.D. -0.7
auxotrophy YNB Glucose +0.1 N.D. -0.8 N.D. -0.7
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.6 N.D. -1.1
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.6 N.D. -0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. -0.4 N.D. -0.3
growth on oleic acid YNB Glucose -0.1 N.D. -0.3 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.8 N.D. -1.4
growth on ricinoleic acid YNB Glucose +0.0 N.D. -0.9 N.D. +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -0.8 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.1 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. -0.5 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. -0.6 N.D. +0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. -0.1 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.5 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.0 N.D. +0.2
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.3 N.D. +0.7
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. +0.2 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. +0.7 N.D. -0.1
growth on ypd YPD -0.2 N.D. +0.1 N.D. +1.6
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