Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10179 and 10180 are separated by 262 nucleotides10180 and 10181 are separated by 970 nucleotides10181 and 10182 are separated by 561 nucleotides10182 and 10183 are separated by 1466 nucleotides mRNA_1811: 10179 - HMMPfam-Centrosome microtubule-binding domain of Cep57-PF06657, at 261,573 to 264,889 10179 mRNA_1812: 10180 - Hypothetical Protein, at 265,152 to 268,202 10180 mRNA_1813: 10181 - K04352 RASA1, RASGAP Ras GTPase-activating protein 1, at 269,173 to 274,604 10181 mRNA_1814: 10182 - K00858 ppnK, NADK NAD+ kinase, at 275,166 to 278,698 10182 mRNA_1815: 10183 - K20777 EXD2 exonuclease 3'-5' domain-containing protein 2, at 280,165 to 281,761 10183
Group Condition mRNA_1811 mRNA_1812 mRNA_1813 mRNA_1814 mRNA_1815
10179 10180 10181 10182 10183
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 -0.1 -1.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 +0.1 -0.1 -0.4 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.8 -0.0 -0.2 +0.0 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.0 +0.1 -0.7 -0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.3 +0.1 -0.2 +0.0
growth on ypd YPD +0.6 -0.2 -0.5 -0.3 +0.2
growth on oleic acid YNB Glucose -0.2 +0.1 +0.2 -0.4 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.3 -0.2 +0.7 +0.2
auxotrophy Dropout Complete +0.4 -0.2 +0.0 -0.6 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.8 +0.1 -0.2 +0.4 +0.5
ypd buoyancy 40 hrs High Buoyancy -0.5 -0.0 +0.0 +0.6 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 -0.1 -0.1 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.3 +0.3 -0.4 +0.3
growth on ricinoleic acid YNB Glucose -0.5 +0.2 +0.1 +0.0 +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.5 -0.2 +0.4 +0.4 +0.1
auxotrophy YNB Glucose +0.8 -0.3 +0.1 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.9 +0.2 +0.4 +0.1 +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 +0.2 -0.1 +0.3 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 +0.2 +0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.0 +0.1 +0.4 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 -0.0 +0.1 +0.3 +0.2
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