Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10181 and 10182 are separated by 561 nucleotides10182 and 10183 are separated by 1466 nucleotides10183 and 10184 are separated by 739 nucleotides10184 and 10185 are separated by 357 nucleotides mRNA_1813: 10181 - K04352 RASA1, RASGAP Ras GTPase-activating protein 1, at 269,173 to 274,604 10181 mRNA_1814: 10182 - K00858 ppnK, NADK NAD+ kinase, at 275,166 to 278,698 10182 mRNA_1815: 10183 - K20777 EXD2 exonuclease 3'-5' domain-containing protein 2, at 280,165 to 281,761 10183 mRNA_1816: 10184 - K08178 JEN MFS transporter, SHS family, lactate transporter, at 282,501 to 284,889 10184 mRNA_1817: 10185 - HMMPfam-Ring finger domain-PF13639,ProSitePatterns-EGF-like domain signature 1.-PS00022,ProSiteProfiles-Zinc finger RING-type profile.-PS50089,ProSiteProfiles-Zinc finger SWIM-type profile.-PS50966,SUPERFAMILY--SSF57850, at 285,247 to 286,558 10185
Group Condition mRNA_1813 mRNA_1814 mRNA_1815 mRNA_1816 mRNA_1817
10181 10182 10183 10184 10185
auxotrophy YNB Glucose +0.1 -0.3 +0.1 +0.4 -2.6
auxotrophy Dropout Complete +0.0 -0.6 +0.3 +0.3 -2.2
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.1 +0.4 +0.3 -2.4
growth on oleic acid YNB Oleic Acid +0.1 -0.2 +0.0 -0.3 -1.4
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -1.3 -0.4 +0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.4 +0.3 +0.3 -1.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.7 +0.2 -0.4 -1.7
growth on oleic acid YNB Glucose +0.2 -0.4 +0.3 -0.3 -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.4 +0.5 -0.1 -1.9
growth on ricinoleic acid YNB Glucose +0.1 +0.0 +0.5 +0.2 -1.8
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.7 -0.2 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.0 +0.1 -0.4 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.6 -0.0 -0.0 -1.4
growth on ypd YPD -0.5 -0.3 +0.2 -0.4 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.4 -0.1 +0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.4 +0.5 +0.1 -0.9
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.3 +0.2 +0.0 -0.7
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.2 +0.0 -0.0 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.3 +0.4 -0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.1 +0.6 -0.3 -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.4 +0.1 -0.2 -0.4
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