Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10188 and 10189 are separated by 32 nucleotides10189 and 10190 are separated by 697 nucleotides10190 and 10191 are separated by 1982 nucleotides10191 and 10192 are separated by 294 nucleotides mRNA_1820: 10188 - Hypothetical Protein, at 290,979 to 291,681 10188 mRNA_1821: 10189 - HMMPfam-Domain of unknown function (DUF2427)-PF10348,HMMPfam-Protein of unknown function (Ytp1)-PF10355, at 291,714 to 294,112 10189 mRNA_1822: 10190 - KOG0627 Heat shock transcription factor, at 294,810 to 296,545 10190 mRNA_1823: 10191 - KOG3783 Uncharacterized conserved protein, at 298,528 to 301,287 10191 mRNA_1824: 10192 - Hypothetical Protein, at 301,582 to 303,988 10192
Group Condition mRNA_1820 mRNA_1821 mRNA_1822 mRNA_1823 mRNA_1824
10188 10189 10190 10191 10192
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.5 -0.4 -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.0 -0.7 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.4 -0.5 -0.3 +0.0
auxotrophy Dropout Complete +0.1 +0.0 -0.4 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.2 -0.4 -0.5 -0.5
growth on oleic acid YNB Glucose -0.1 -0.0 -0.2 +0.5 -0.4
growth on ypd YPD -0.1 +0.3 +0.0 -0.2 -0.3
growth on ricinoleic acid YNB Glucose +0.1 +0.3 -0.1 +0.1 -0.6
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.0 +0.0 +0.2 -0.3
auxotrophy YNB Glucose +0.2 +0.1 -0.2 -0.0 -0.2
growth on oleic acid YNB Oleic Acid -0.1 +0.1 +0.0 +0.3 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 +0.1 +0.2 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.0 -0.2 -0.1 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.2 -0.1 +0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.0 -0.2 +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.0 +0.3 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.0 +0.0 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.6 -0.2 +0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.0 +0.4 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.1 +0.5 -0.2 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 +0.3 +0.1 +0.3
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