Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10197 and 10198 are separated by 279 nucleotides10198 and 10199 are separated by 295 nucleotides10199 and 10200 are separated by 450 nucleotides10200 and 10201 are separated by 567 nucleotides mRNA_1829: 10197 - K20238 E2.1.1.317 sphingolipid C9-methyltransferase, at 314,887 to 317,031 10197 mRNA_1830: 10198 - K00809 DHPS, dys deoxyhypusine synthase, at 317,311 to 318,689 10198 mRNA_1831: 10199 - KOG3274 Uncharacterized conserved protein, AMMECR1, at 318,985 to 319,920 10199 mRNA_1832: 10200 - K11188 PRDX6 peroxiredoxin 6, 1-Cys peroxiredoxin, at 320,371 to 321,858 10200 mRNA_1833: 10201 - Hypothetical Protein, at 322,426 to 322,877 10201
Group Condition mRNA_1829 mRNA_1830 mRNA_1831 mRNA_1832 mRNA_1833
10197 10198 10199 10200 10201
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. -1.4 +0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy -1.3 N.D. -0.5 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. -0.8 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.4 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. -0.4 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. +0.1 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.9 N.D. +0.3 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.8 +0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.3 +0.5 N.D.
growth on oleic acid YNB Glucose -0.0 N.D. -0.1 +0.2 N.D.
growth on oleic acid YNB Oleic Acid +0.7 N.D. -0.5 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.2 +0.8 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. +0.7 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -0.0 +0.4 N.D.
growth on ypd YPD +0.4 N.D. +0.1 -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. +0.4 +0.5 N.D.
auxotrophy YNB Glucose -0.1 N.D. +0.3 +0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 N.D. -0.2 +0.2 N.D.
auxotrophy Dropout Complete +0.2 N.D. +0.4 +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. +0.3 +0.2 N.D.
growth on ricinoleic acid YNB Glucose +1.1 N.D. -0.2 +0.2 N.D.
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