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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10211 and 10212 are separated by 230 nucleotides
10212 and 10213 are separated by 493 nucleotides
10213 and 10214 are separated by 244 nucleotides
10214 and 10215 are separated by 575 nucleotides
mRNA_1843: 10211 - Hypothetical Protein, at 347,522 to 353,659
10211
mRNA_1844: 10212 - K16066 ydfG 3-hydroxy acid dehydrogenase / malonic semialdehyde reductase, at 353,890 to 355,492
10212
mRNA_1845: 10213 - K06127 COQ5 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, at 355,986 to 356,917
10213
mRNA_1846: 10214 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 357,162 to 359,735
10214
mRNA_1847: 10215 - BLAST GDP-fucose protein O-fucosyltransferase [Rhizopogon v..., at 360,311 to 362,309
10215
Group
Condition
mRNA
_1843
mRNA
_1844
mRNA
_1845
mRNA
_1846
mRNA
_1847
10211
10212
10213
10214
10215
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.2
-1.3
N.D.
+0.5
+0.0
ypd buoyancy
40 hrs Median Buoyancy
-0.0
-0.4
N.D.
+0.0
+0.0
ypd buoyancy
40 hrs High Buoyancy
+0.1
-0.2
N.D.
-0.3
-0.0
growth on oleic acid
YNB Oleic Acid
-0.0
+0.3
N.D.
-0.0
-0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
+0.4
N.D.
-0.2
-0.2
auxotrophy
Dropout Complete
-0.1
-0.0
N.D.
+0.4
+0.0
auxotrophy
YNB Glucose 75mM Methionine
-0.0
-0.1
N.D.
+0.5
+0.0
growth on oleic acid
YNB Glucose
+0.1
+0.4
N.D.
-0.0
-0.0
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
+0.1
N.D.
+0.2
+0.0
auxotrophy
YNB Glucose
-0.3
+0.3
N.D.
+0.5
+0.1
facs on lipid accumulation
Sorted No BODIPY Gate
-0.0
+0.1
N.D.
+0.5
+0.3
growth on ypd
YPD
+0.1
+0.6
N.D.
+0.0
+0.2
auxotrophy
YNB Glucose 75mM Arginine
-0.1
+0.4
N.D.
+0.6
+0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
+0.5
N.D.
+0.7
-0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.9
+0.4
N.D.
-0.0
-0.3
ypd buoyancy
40 hrs Low Buoyancy
+0.2
+0.9
N.D.
+0.1
-0.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
+0.7
N.D.
+0.5
+0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
+1.4
N.D.
+0.2
-0.1
growth on ricinoleic acid
YNB Glucose
-0.0
+0.7
N.D.
+0.9
+0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.1
+1.4
N.D.
+0.9
+0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.2
+1.4
N.D.
+0.9
+0.2
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