Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10211 and 10212 are separated by 230 nucleotides10212 and 10213 are separated by 493 nucleotides10213 and 10214 are separated by 244 nucleotides10214 and 10215 are separated by 575 nucleotides mRNA_1843: 10211 - Hypothetical Protein, at 347,522 to 353,659 10211 mRNA_1844: 10212 - K16066 ydfG 3-hydroxy acid dehydrogenase / malonic semialdehyde reductase, at 353,890 to 355,492 10212 mRNA_1845: 10213 - K06127 COQ5 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, at 355,986 to 356,917 10213 mRNA_1846: 10214 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 357,162 to 359,735 10214 mRNA_1847: 10215 - BLAST GDP-fucose protein O-fucosyltransferase [Rhizopogon v..., at 360,311 to 362,309 10215
Group Condition mRNA_1843 mRNA_1844 mRNA_1845 mRNA_1846 mRNA_1847
10211 10212 10213 10214 10215
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -1.3 N.D. +0.5 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.4 N.D. +0.0 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.2 N.D. -0.3 -0.0
growth on oleic acid YNB Oleic Acid -0.0 +0.3 N.D. -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.4 N.D. -0.2 -0.2
auxotrophy Dropout Complete -0.1 -0.0 N.D. +0.4 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.1 N.D. +0.5 +0.0
growth on oleic acid YNB Glucose +0.1 +0.4 N.D. -0.0 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 N.D. +0.2 +0.0
auxotrophy YNB Glucose -0.3 +0.3 N.D. +0.5 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.1 N.D. +0.5 +0.3
growth on ypd YPD +0.1 +0.6 N.D. +0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.4 N.D. +0.6 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.5 N.D. +0.7 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.9 +0.4 N.D. -0.0 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.9 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.7 N.D. +0.5 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +1.4 N.D. +0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.0 +0.7 N.D. +0.9 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +1.4 N.D. +0.9 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +1.4 N.D. +0.9 +0.2
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