Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10213 and 10214 are separated by 244 nucleotides10214 and 10215 are separated by 575 nucleotides10215 and 10216 are separated by 640 nucleotides10216 and 10217 are separated by 251 nucleotides mRNA_1845: 10213 - K06127 COQ5 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, at 355,986 to 356,917 10213 mRNA_1846: 10214 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 357,162 to 359,735 10214 mRNA_1847: 10215 - BLAST GDP-fucose protein O-fucosyltransferase [Rhizopogon v..., at 360,311 to 362,309 10215 mRNA_1848: 10216 - BLAST PC-Esterase [Xanthophyllomyces dendrorhous], at 362,950 to 364,987 10216 mRNA_1849: 10217 - Hypothetical Protein, at 365,239 to 367,490 10217
Group Condition mRNA_1845 mRNA_1846 mRNA_1847 mRNA_1848 mRNA_1849
10213 10214 10215 10216 10217
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 -0.0 -0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 -0.3 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -0.2 +0.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 -0.1 -0.3 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.2 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.0 +0.0 -0.1
growth on oleic acid YNB Glucose N.D. -0.0 -0.0 +0.0 -0.0
growth on ypd YPD N.D. +0.0 +0.2 -0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.0 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 +0.0 -0.1 +0.1
auxotrophy Dropout Complete N.D. +0.4 +0.0 -0.2 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.5 +0.0 -0.2 +0.0
auxotrophy YNB Glucose N.D. +0.5 +0.1 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.7 -0.1 -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.6 +0.1 -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.5 +0.3 -0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.9 +0.2 -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.9 +0.1 -0.1 -0.0
growth on ricinoleic acid YNB Glucose N.D. +0.9 +0.1 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 +0.1 +0.1 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.5 +0.0 +0.5 +0.1
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