Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10214 and 10215 are separated by 575 nucleotides10215 and 10216 are separated by 640 nucleotides10216 and 10217 are separated by 251 nucleotides10217 and 10218 are separated by 1084 nucleotides mRNA_1846: 10214 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 357,162 to 359,735 10214 mRNA_1847: 10215 - BLAST GDP-fucose protein O-fucosyltransferase [Rhizopogon v..., at 360,311 to 362,309 10215 mRNA_1848: 10216 - BLAST PC-Esterase [Xanthophyllomyces dendrorhous], at 362,950 to 364,987 10216 mRNA_1849: 10217 - Hypothetical Protein, at 365,239 to 367,490 10217 mRNA_1850: 10218 - K00311 ETFDH electron-transferring-flavoprotein dehydrogenase, at 368,575 to 371,026 10218
Group Condition mRNA_1846 mRNA_1847 mRNA_1848 mRNA_1849 mRNA_1850
10214 10215 10216 10217 10218
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 +0.1 -0.1 -0.0 -3.7
growth on oleic acid YNB Oleic Acid -0.0 -0.2 +0.1 -0.1 -2.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.9 +0.2 -0.1 -0.1 -2.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.2 +0.0 +0.1 -1.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.3 +0.1 -0.3 -0.3
growth on oleic acid YNB Glucose -0.0 -0.0 +0.0 -0.0 -0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 -0.3 +0.0 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.0 -0.1 +0.1 -0.7
facs on lipid accumulation Sorted No BODIPY Gate +0.5 +0.3 -0.2 +0.2 -1.0
auxotrophy YNB Glucose +0.5 +0.1 -0.1 -0.0 -0.6
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.0 +0.0 -0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.0 -0.2 +0.0 -0.5
growth on ypd YPD +0.0 +0.2 -0.2 -0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.0 -0.1 -0.0 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 +0.0 +0.5 +0.1 -1.2
auxotrophy YNB Glucose 75mM Arginine +0.6 +0.1 -0.2 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 -0.1 -0.3 +0.1 -0.1
auxotrophy Dropout Complete +0.4 +0.0 -0.2 +0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.0 +0.1 -0.1 +0.5
growth on ricinoleic acid YNB Glucose +0.9 +0.1 +0.1 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.1 +0.1 +0.4 -0.2
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