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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10214 and 10215 are separated by 575 nucleotides
10215 and 10216 are separated by 640 nucleotides
10216 and 10217 are separated by 251 nucleotides
10217 and 10218 are separated by 1084 nucleotides
mRNA_1846: 10214 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 357,162 to 359,735
10214
mRNA_1847: 10215 - BLAST GDP-fucose protein O-fucosyltransferase [Rhizopogon v..., at 360,311 to 362,309
10215
mRNA_1848: 10216 - BLAST PC-Esterase [Xanthophyllomyces dendrorhous], at 362,950 to 364,987
10216
mRNA_1849: 10217 - Hypothetical Protein, at 365,239 to 367,490
10217
mRNA_1850: 10218 - K00311 ETFDH electron-transferring-flavoprotein dehydrogenase, at 368,575 to 371,026
10218
Group
Condition
mRNA
_1846
mRNA
_1847
mRNA
_1848
mRNA
_1849
mRNA
_1850
10214
10215
10216
10217
10218
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.9
+0.1
-0.1
-0.0
-3.7
growth on oleic acid
YNB Oleic Acid
-0.0
-0.2
+0.1
-0.1
-2.5
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.9
+0.2
-0.1
-0.1
-2.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
-0.2
+0.0
+0.1
-1.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.0
-0.3
+0.1
-0.3
-0.3
growth on oleic acid
YNB Glucose
-0.0
-0.0
+0.0
-0.0
-0.7
facs on lipid accumulation
Sorted High BODIPY Gate
+0.2
-0.1
-0.3
+0.0
-0.4
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.2
+0.0
-0.1
+0.1
-0.7
facs on lipid accumulation
Sorted No BODIPY Gate
+0.5
+0.3
-0.2
+0.2
-1.0
auxotrophy
YNB Glucose
+0.5
+0.1
-0.1
-0.0
-0.6
ypd buoyancy
40 hrs Low Buoyancy
+0.1
-0.0
+0.0
-0.1
-0.2
auxotrophy
YNB Glucose 75mM Methionine
+0.5
+0.0
-0.2
+0.0
-0.5
growth on ypd
YPD
+0.0
+0.2
-0.2
-0.1
-0.2
ypd buoyancy
40 hrs High Buoyancy
-0.3
-0.0
-0.1
-0.0
+0.4
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.5
+0.0
+0.5
+0.1
-1.2
auxotrophy
YNB Glucose 75mM Arginine
+0.6
+0.1
-0.2
+0.1
-0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.7
-0.1
-0.3
+0.1
-0.1
auxotrophy
Dropout Complete
+0.4
+0.0
-0.2
+0.0
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.0
+0.0
+0.1
-0.1
+0.5
growth on ricinoleic acid
YNB Glucose
+0.9
+0.1
+0.1
-0.1
-0.3
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.5
+0.1
+0.1
+0.4
-0.2
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