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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10217 and 10218 are separated by 1084 nucleotides
10218 and 10219 are separated by 721 nucleotides
10219 and 10220 are separated by 82 nucleotides
10220 and 10221 are separated by 511 nucleotides
mRNA_1849: 10217 - Hypothetical Protein, at 365,239 to 367,490
10217
mRNA_1850: 10218 - K00311 ETFDH electron-transferring-flavoprotein dehydrogenase, at 368,575 to 371,026
10218
mRNA_1851: 10219 - BLAST cell wall surface anchor family protein [Moniliop..., at 371,748 to 376,200
10219
mRNA_1852: 10220 - K06133 LYS5, acpT 4'-phosphopantetheinyl transferase, at 376,283 to 377,328
10220
mRNA_1853: 10221 - KOG4147 Uncharacterized conserved protein, at 377,840 to 378,489
10221
Group
Condition
mRNA
_1849
mRNA
_1850
mRNA
_1851
mRNA
_1852
mRNA
_1853
10217
10218
10219
10220
10221
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.0
-3.7
+0.1
N.D.
N.D.
growth on oleic acid
YNB Oleic Acid
-0.1
-2.5
+0.0
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
-2.3
+0.3
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
-1.1
+0.0
N.D.
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
-0.3
-0.3
-0.4
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.1
-1.2
+0.1
N.D.
N.D.
auxotrophy
YNB Glucose
-0.0
-0.6
-0.2
N.D.
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
+0.2
-1.0
+0.0
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
-0.1
-0.3
-0.3
N.D.
N.D.
growth on oleic acid
YNB Glucose
-0.0
-0.7
+0.0
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
+0.0
-0.5
-0.1
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
-0.7
+0.1
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
-0.1
-0.2
-0.2
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
+0.0
-0.4
+0.0
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
+0.1
-0.3
+0.1
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.1
-0.1
-0.1
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
-0.0
+0.4
-0.3
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.4
-0.2
-0.1
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.5
-0.2
N.D.
N.D.
growth on ypd
YPD
-0.1
-0.2
+0.3
N.D.
N.D.
auxotrophy
Dropout Complete
+0.0
+0.1
+0.0
N.D.
N.D.
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