Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10218 and 10219 are separated by 721 nucleotides10219 and 10220 are separated by 82 nucleotides10220 and 10221 are separated by 511 nucleotides10221 and 10222 are separated by 161 nucleotides mRNA_1850: 10218 - K00311 ETFDH electron-transferring-flavoprotein dehydrogenase, at 368,575 to 371,026 10218 mRNA_1851: 10219 - BLAST cell wall surface anchor family protein [Moniliop..., at 371,748 to 376,200 10219 mRNA_1852: 10220 - K06133 LYS5, acpT 4'-phosphopantetheinyl transferase, at 376,283 to 377,328 10220 mRNA_1853: 10221 - KOG4147 Uncharacterized conserved protein, at 377,840 to 378,489 10221 mRNA_1854: 10222 - Hypothetical Protein, at 378,651 to 380,549 10222
Group Condition mRNA_1850 mRNA_1851 mRNA_1852 mRNA_1853 mRNA_1854
10218 10219 10220 10221 10222
growth on ricinoleic acid YNB Ricinoleic Acid -3.7 +0.1 N.D. N.D. -0.0
growth on oleic acid YNB Oleic Acid -2.5 +0.0 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -2.3 +0.3 N.D. N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.4 N.D. N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -1.2 +0.1 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.1 +0.0 N.D. N.D. +0.0
growth on oleic acid YNB Glucose -0.7 +0.0 N.D. N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate -1.0 +0.0 N.D. N.D. +0.2
auxotrophy YNB Glucose -0.6 -0.2 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.3 -0.3 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 +0.1 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine -0.5 -0.1 N.D. N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.0 N.D. N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.2 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.1 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 N.D. N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.3 N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.1 N.D. N.D. +0.1
auxotrophy Dropout Complete +0.1 +0.0 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.2 N.D. N.D. -0.0
growth on ypd YPD -0.2 +0.3 N.D. N.D. +0.1
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