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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10219 and 10220 are separated by 82 nucleotides
10220 and 10221 are separated by 511 nucleotides
10221 and 10222 are separated by 161 nucleotides
10222 and 10223 are separated by 409 nucleotides
mRNA_1851: 10219 - BLAST cell wall surface anchor family protein [Moniliop..., at 371,748 to 376,200
10219
mRNA_1852: 10220 - K06133 LYS5, acpT 4'-phosphopantetheinyl transferase, at 376,283 to 377,328
10220
mRNA_1853: 10221 - KOG4147 Uncharacterized conserved protein, at 377,840 to 378,489
10221
mRNA_1854: 10222 - Hypothetical Protein, at 378,651 to 380,549
10222
mRNA_1855: 10223 - BLAST secreted protein [Moesziomyces antarcticus], at 380,959 to 382,150
10223
Group
Condition
mRNA
_1851
mRNA
_1852
mRNA
_1853
mRNA
_1854
mRNA
_1855
10219
10220
10221
10222
10223
ypd buoyancy
40 hrs No Buoyancy Separation
-0.4
N.D.
N.D.
-0.4
-0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.1
N.D.
N.D.
-0.0
-0.8
ypd buoyancy
40 hrs High Buoyancy
-0.3
N.D.
N.D.
-0.2
-0.2
growth on ricinoleic acid
YNB Glucose
-0.3
N.D.
N.D.
+0.0
-0.3
growth on oleic acid
YNB Glucose
+0.0
N.D.
N.D.
-0.2
-0.4
ypd buoyancy
40 hrs Median Buoyancy
-0.2
N.D.
N.D.
-0.0
-0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.1
N.D.
N.D.
-0.0
-0.5
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.1
N.D.
N.D.
-0.1
-0.2
growth on oleic acid
YNB Oleic Acid
+0.0
N.D.
N.D.
-0.1
-0.3
ypd buoyancy
40 hrs Low Buoyancy
-0.2
N.D.
N.D.
+0.0
-0.0
auxotrophy
YNB Glucose
-0.2
N.D.
N.D.
+0.1
-0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.3
N.D.
N.D.
+0.1
-0.4
facs on lipid accumulation
Sorted No BODIPY Gate
+0.0
N.D.
N.D.
+0.2
-0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.0
N.D.
N.D.
+0.0
-0.0
facs on lipid accumulation
Sorted High BODIPY Gate
+0.0
N.D.
N.D.
+0.0
-0.0
auxotrophy
Dropout Complete
+0.0
N.D.
N.D.
+0.1
-0.0
auxotrophy
YNB Glucose 75mM Methionine
-0.1
N.D.
N.D.
+0.2
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.1
N.D.
N.D.
+0.1
+0.3
auxotrophy
YNB Glucose 75mM Arginine
+0.1
N.D.
N.D.
+0.1
+0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
N.D.
N.D.
+0.0
+0.3
growth on ypd
YPD
+0.3
N.D.
N.D.
+0.1
+0.8
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