Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10219 and 10220 are separated by 82 nucleotides10220 and 10221 are separated by 511 nucleotides10221 and 10222 are separated by 161 nucleotides10222 and 10223 are separated by 409 nucleotides mRNA_1851: 10219 - BLAST cell wall surface anchor family protein [Moniliop..., at 371,748 to 376,200 10219 mRNA_1852: 10220 - K06133 LYS5, acpT 4'-phosphopantetheinyl transferase, at 376,283 to 377,328 10220 mRNA_1853: 10221 - KOG4147 Uncharacterized conserved protein, at 377,840 to 378,489 10221 mRNA_1854: 10222 - Hypothetical Protein, at 378,651 to 380,549 10222 mRNA_1855: 10223 - BLAST secreted protein [Moesziomyces antarcticus], at 380,959 to 382,150 10223
Group Condition mRNA_1851 mRNA_1852 mRNA_1853 mRNA_1854 mRNA_1855
10219 10220 10221 10222 10223
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. N.D. -0.4 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. N.D. -0.0 -0.8
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. N.D. -0.2 -0.2
growth on ricinoleic acid YNB Glucose -0.3 N.D. N.D. +0.0 -0.3
growth on oleic acid YNB Glucose +0.0 N.D. N.D. -0.2 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. -0.0 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. N.D. -0.1 -0.2
growth on oleic acid YNB Oleic Acid +0.0 N.D. N.D. -0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. +0.0 -0.0
auxotrophy YNB Glucose -0.2 N.D. N.D. +0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. +0.1 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.0 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. N.D. +0.0 -0.0
auxotrophy Dropout Complete +0.0 N.D. N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. +0.0 +0.3
growth on ypd YPD +0.3 N.D. N.D. +0.1 +0.8
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