Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10220 and 10221 are separated by 511 nucleotides10221 and 10222 are separated by 161 nucleotides10222 and 10223 are separated by 409 nucleotides10223 and 10224 are separated by 184 nucleotides mRNA_1852: 10220 - K06133 LYS5, acpT 4'-phosphopantetheinyl transferase, at 376,283 to 377,328 10220 mRNA_1853: 10221 - KOG4147 Uncharacterized conserved protein, at 377,840 to 378,489 10221 mRNA_1854: 10222 - Hypothetical Protein, at 378,651 to 380,549 10222 mRNA_1855: 10223 - BLAST secreted protein [Moesziomyces antarcticus], at 380,959 to 382,150 10223 mRNA_1856: 10224 - K10863 APTX aprataxin, at 382,335 to 383,661 10224
Group Condition mRNA_1852 mRNA_1853 mRNA_1854 mRNA_1855 mRNA_1856
10220 10221 10222 10223 10224
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.0 -0.8 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 -0.4 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.4 -0.2 -0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.2 -0.4 -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.0 -0.3 -0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.2 -0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.0 -0.5 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.0 +0.3 -0.8
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.1 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 -0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 -0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.0 -0.0 -0.2
auxotrophy Dropout Complete N.D. N.D. +0.1 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.0 -0.0 -0.1
auxotrophy YNB Glucose N.D. N.D. +0.1 -0.0 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.0 -0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.0 -0.0 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.2 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 +0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 +0.2 +0.2
growth on ypd YPD N.D. N.D. +0.1 +0.8 +0.2
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