Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10222 and 10223 are separated by 409 nucleotides10223 and 10224 are separated by 184 nucleotides10224 and 10225 are separated by 288 nucleotides10225 and 10226 are separated by 712 nucleotides mRNA_1854: 10222 - Hypothetical Protein, at 378,651 to 380,549 10222 mRNA_1855: 10223 - BLAST secreted protein [Moesziomyces antarcticus], at 380,959 to 382,150 10223 mRNA_1856: 10224 - K10863 APTX aprataxin, at 382,335 to 383,661 10224 mRNA_1857: 10225 - KOG2533 Permease of the major facilitator superfamily, at 383,950 to 386,547 10225 mRNA_1858: 10226 - K19245 JLP1 sulfonate dioxygenase, at 387,260 to 388,608 10226
Group Condition mRNA_1854 mRNA_1855 mRNA_1856 mRNA_1857 mRNA_1858
10222 10223 10224 10225 10226
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.8 -0.3 -0.6 -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 -0.0 -0.8 -0.2
growth on oleic acid YNB Glucose -0.2 -0.4 -0.2 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.0 -0.1 -0.4 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.5 -0.1 +0.6 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.4 -0.6 +0.4 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.3 -0.8 -0.1 +0.1
growth on ricinoleic acid YNB Glucose +0.0 -0.3 -0.5 +0.5 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.2 -0.2 +0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.2 -0.2 +0.0 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.0 -0.2 -0.4 +0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.3 -0.0 +0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.2 -0.2 +0.4 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.3 +0.0 -0.3 +0.0
auxotrophy Dropout Complete +0.1 -0.0 -0.2 -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.2 +0.2 -0.2 +0.3
auxotrophy YNB Glucose +0.1 -0.0 -0.0 -0.2 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 +0.2 -0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.1 +0.1 -0.1 +0.3
growth on ypd YPD +0.1 +0.8 +0.2 -0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.0 +0.2 +0.1 +0.5
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