Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10226 and 10227 are separated by 248 nucleotides10227 and 10228 are separated by 271 nucleotides10228 and 10229 are separated by 141 nucleotides10229 and 10230 are separated by 423 nucleotides mRNA_1858: 10226 - K19245 JLP1 sulfonate dioxygenase, at 387,260 to 388,608 10226 mRNA_1859: 10227 - SUPERFAMILY--SSF57997, at 388,857 to 391,098 10227 mRNA_1860: 10228 - K02259 COX15 cytochrome c oxidase assembly protein subunit 15, at 391,370 to 393,300 10228 mRNA_1861: 10229 - KOG3348 BolA (bacterial stress-induced morphogen)-related protein, at 393,442 to 393,978 10229 mRNA_1862: 10230 - Interpro DNA replication factor Cdt1 C-terminal domain 0, at 394,402 to 396,759 10230
Group Condition mRNA_1858 mRNA_1859 mRNA_1860 mRNA_1861 mRNA_1862
10226 10227 10228 10229 10230
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 +0.0 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.1 N.D. N.D. -0.2
growth on oleic acid YNB Glucose -0.2 +0.2 N.D. N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 +0.1 N.D. N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. N.D. -0.2
growth on ricinoleic acid YNB Glucose -0.2 +0.0 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.0 N.D. N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.4 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.2 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.0 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.1 N.D. N.D. +0.1
auxotrophy Dropout Complete +0.3 -0.2 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.0 N.D. N.D. -0.2
growth on ypd YPD +0.1 +0.1 N.D. N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.3 N.D. N.D. +0.1
auxotrophy YNB Glucose +0.5 -0.2 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.2 N.D. N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.5 +0.0 N.D. N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.3 N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.4 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.2 N.D. N.D. +0.7
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