Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10228 and 10229 are separated by 141 nucleotides10229 and 10230 are separated by 423 nucleotides10230 and 10231 are separated by 133 nucleotides10231 and 10232 are separated by 382 nucleotides mRNA_1860: 10228 - K02259 COX15 cytochrome c oxidase assembly protein subunit 15, at 391,370 to 393,300 10228 mRNA_1861: 10229 - KOG3348 BolA (bacterial stress-induced morphogen)-related protein, at 393,442 to 393,978 10229 mRNA_1862: 10230 - Interpro DNA replication factor Cdt1 C-terminal domain 0, at 394,402 to 396,759 10230 mRNA_1863: 10231 - HMMPfam-Metallo-beta-lactamase superfamily-PF00753,SMART-Metallo-beta-lactamase superfamily-SM00849,SUPERFAMILY--SSF56281, at 396,893 to 397,973 10231 mRNA_1864: 10232 - SUPERFAMILY--SSF53300, at 398,356 to 399,523 10232
Group Condition mRNA_1860 mRNA_1861 mRNA_1862 mRNA_1863 mRNA_1864
10228 10229 10230 10231 10232
growth on ypd YPD N.D. N.D. -0.0 -0.2 -1.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.3 -0.6 -1.4
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.0 -0.0 -1.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 -0.1 -1.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.2 -0.2 -0.7
growth on oleic acid YNB Glucose N.D. N.D. -0.4 -0.1 -0.6
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.0 -0.0 -0.9
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.1 -0.6
auxotrophy YNB Glucose N.D. N.D. -0.1 -0.3 -0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 -0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 -0.8 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 -0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.3 +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.5 -0.0 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 -0.5 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.2 -1.0 +1.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 +0.1 +0.1
auxotrophy Dropout Complete N.D. N.D. -0.1 +0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.7 -0.3 +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 -0.6 +1.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 -0.4 +1.7
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