Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10229 and 10230 are separated by 423 nucleotides10230 and 10231 are separated by 133 nucleotides10231 and 10232 are separated by 382 nucleotides10232 and 10233 are separated by 214 nucleotides mRNA_1861: 10229 - KOG3348 BolA (bacterial stress-induced morphogen)-related protein, at 393,442 to 393,978 10229 mRNA_1862: 10230 - Interpro DNA replication factor Cdt1 C-terminal domain 0, at 394,402 to 396,759 10230 mRNA_1863: 10231 - HMMPfam-Metallo-beta-lactamase superfamily-PF00753,SMART-Metallo-beta-lactamase superfamily-SM00849,SUPERFAMILY--SSF56281, at 396,893 to 397,973 10231 mRNA_1864: 10232 - SUPERFAMILY--SSF53300, at 398,356 to 399,523 10232 mRNA_1865: 10233 - K02729 PSMA5 20S proteasome subunit alpha 5, at 399,738 to 401,124 10233
Group Condition mRNA_1861 mRNA_1862 mRNA_1863 mRNA_1864 mRNA_1865
10229 10230 10231 10232 10233
growth on ypd YPD N.D. -0.0 -0.2 -1.9 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 -0.6 -1.4 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.0 -0.0 -1.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 -0.1 -1.4 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.2 -0.7 N.D.
growth on oleic acid YNB Glucose N.D. -0.4 -0.1 -0.6 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.0 -0.9 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.1 -0.6 N.D.
auxotrophy YNB Glucose N.D. -0.1 -0.3 -0.4 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.2 -0.3 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 -0.8 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.2 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 +0.1 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 -0.0 +0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 -0.5 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 -1.0 +1.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 +0.1 +0.1 N.D.
auxotrophy Dropout Complete N.D. -0.1 +0.2 +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 -0.3 +0.9 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.6 +1.8 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 -0.4 +1.7 N.D.
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