Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10232 and 10233 are separated by 214 nucleotides10233 and 10234 are separated by 162 nucleotides10234 and 10235 are separated by 445 nucleotides10235 and 10236 are separated by 123 nucleotides mRNA_1864: 10232 - SUPERFAMILY--SSF53300, at 398,356 to 399,523 10232 mRNA_1865: 10233 - K02729 PSMA5 20S proteasome subunit alpha 5, at 399,738 to 401,124 10233 mRNA_1866: 10234 - KOG4455 Uncharacterized conserved protein, at 401,287 to 402,071 10234 mRNA_1867: 10235 - HMMPfam-von Willebrand factor type A domain-PF00092,ProSiteProfiles-VWFA domain profile.-PS50234,SMART-von Willebrand factor (vWF) type A domain-SM00327,SUPERFAMILY--SSF53300, at 402,517 to 403,659 10235 mRNA_1868: 10236 - K11569 DAD4, HSK2 DASH complex subunit DAD4, at 403,783 to 404,255 10236
Group Condition mRNA_1864 mRNA_1865 mRNA_1866 mRNA_1867 mRNA_1868
10232 10233 10234 10235 10236
growth on ricinoleic acid YNB Glucose -1.5 N.D. -0.5 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -1.4 N.D. -0.4 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.9 N.D. +0.3 -0.9 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.4 N.D. -0.2 +0.2 N.D.
growth on ypd YPD -1.9 N.D. +0.4 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.6 N.D. +0.3 -0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.1 -0.8 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.7 +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.0 -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.9 N.D. -0.7 -0.3 N.D.
auxotrophy YNB Glucose -0.4 N.D. +0.5 -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.7 N.D. +0.8 +0.1 N.D.
growth on oleic acid YNB Glucose -0.6 N.D. +0.4 +0.4 N.D.
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.6 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. +0.8 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.3 N.D. -0.3 -0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. +0.5 +0.0 N.D.
auxotrophy Dropout Complete +0.2 N.D. +0.4 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.7 N.D. +0.6 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.7 N.D. -0.5 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.8 N.D. -0.3 +0.5 N.D.
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