Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10233 and 10234 are separated by 162 nucleotides10234 and 10235 are separated by 445 nucleotides10235 and 10236 are separated by 123 nucleotides10236 and 10237 are separated by 386 nucleotides mRNA_1865: 10233 - K02729 PSMA5 20S proteasome subunit alpha 5, at 399,738 to 401,124 10233 mRNA_1866: 10234 - KOG4455 Uncharacterized conserved protein, at 401,287 to 402,071 10234 mRNA_1867: 10235 - HMMPfam-von Willebrand factor type A domain-PF00092,ProSiteProfiles-VWFA domain profile.-PS50234,SMART-von Willebrand factor (vWF) type A domain-SM00327,SUPERFAMILY--SSF53300, at 402,517 to 403,659 10235 mRNA_1868: 10236 - K11569 DAD4, HSK2 DASH complex subunit DAD4, at 403,783 to 404,255 10236 mRNA_1869: 10237 - K14570 REX1, REXO1, RNH70 RNA exonuclease 1, at 404,642 to 406,231 10237
Group Condition mRNA_1865 mRNA_1866 mRNA_1867 mRNA_1868 mRNA_1869
10233 10234 10235 10236 10237
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.7 -0.3 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 -0.5 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 -0.8 N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. -0.5 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.7 +0.2 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 -0.9 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 -0.1 N.D. -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 -0.0 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.8 -0.4 N.D. -0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 -0.4 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 +0.3 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.6 -0.4 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 +0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 +0.5 N.D. -0.0
auxotrophy Dropout Complete N.D. +0.4 +0.2 N.D. -0.4
growth on oleic acid YNB Oleic Acid N.D. +0.6 -0.2 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.5 +0.0 N.D. +0.0
growth on oleic acid YNB Glucose N.D. +0.4 +0.4 N.D. -0.2
auxotrophy YNB Glucose N.D. +0.5 -0.3 N.D. +0.5
auxotrophy YNB Glucose 75mM Methionine N.D. +0.8 +0.1 N.D. -0.0
growth on ypd YPD N.D. +0.4 +0.2 N.D. +0.4
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