Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10235 and 10236 are separated by 123 nucleotides10236 and 10237 are separated by 386 nucleotides10237 and 10238 are separated by 519 nucleotides10238 and 10239 are separated by 49 nucleotides mRNA_1867: 10235 - HMMPfam-von Willebrand factor type A domain-PF00092,ProSiteProfiles-VWFA domain profile.-PS50234,SMART-von Willebrand factor (vWF) type A domain-SM00327,SUPERFAMILY--SSF53300, at 402,517 to 403,659 10235 mRNA_1868: 10236 - K11569 DAD4, HSK2 DASH complex subunit DAD4, at 403,783 to 404,255 10236 mRNA_1869: 10237 - K14570 REX1, REXO1, RNH70 RNA exonuclease 1, at 404,642 to 406,231 10237 mRNA_1870: 10238 - K18043 OCA1 tyrosine-protein phosphatase OCA1, at 406,751 to 407,606 10238 mRNA_1871: 10239 - K14855 RSA4, NLE1 ribosome assembly protein 4, at 407,656 to 409,995 10239
Group Condition mRNA_1867 mRNA_1868 mRNA_1869 mRNA_1870 mRNA_1871
10235 10236 10237 10238 10239
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -0.5 -2.6 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. -0.8 -0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. -0.1 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -0.1 -0.1 N.D.
auxotrophy Dropout Complete +0.2 N.D. -0.4 -0.6 N.D.
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.0 -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.9 N.D. +0.1 +0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.5 +0.2 N.D.
auxotrophy YNB Glucose -0.3 N.D. +0.5 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.0 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. -0.2 +0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. -0.1 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. -0.2 +0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. +0.0 -0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.2 N.D. -0.1 +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. +0.1 +0.2 N.D.
growth on ypd YPD +0.2 N.D. +0.4 +0.1 N.D.
growth on oleic acid YNB Glucose +0.4 N.D. -0.2 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. +0.1 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. -0.0 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.2 +1.2 N.D.
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