Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10236 and 10237 are separated by 386 nucleotides10237 and 10238 are separated by 519 nucleotides10238 and 10239 are separated by 49 nucleotides10239 and 10240 are separated by 242 nucleotides mRNA_1868: 10236 - K11569 DAD4, HSK2 DASH complex subunit DAD4, at 403,783 to 404,255 10236 mRNA_1869: 10237 - K14570 REX1, REXO1, RNH70 RNA exonuclease 1, at 404,642 to 406,231 10237 mRNA_1870: 10238 - K18043 OCA1 tyrosine-protein phosphatase OCA1, at 406,751 to 407,606 10238 mRNA_1871: 10239 - K14855 RSA4, NLE1 ribosome assembly protein 4, at 407,656 to 409,995 10239 mRNA_1872: 10240 - K14566 UTP24, FCF1 U3 small nucleolar RNA-associated protein 24, at 410,238 to 411,340 10240
Group Condition mRNA_1868 mRNA_1869 mRNA_1870 mRNA_1871 mRNA_1872
10236 10237 10238 10239 10240
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 -2.6 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.8 -0.6 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.4 -0.6 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.4 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 -0.4 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.5 +0.2 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 -0.1 N.D. N.D.
auxotrophy YNB Glucose N.D. +0.5 -0.5 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.1 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 +0.4 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.4 N.D. N.D.
growth on oleic acid YNB Glucose N.D. -0.2 +0.5 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.2 N.D. N.D.
growth on ypd YPD N.D. +0.4 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 +0.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 +1.2 N.D. N.D.
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