Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10238 and 10239 are separated by 49 nucleotides10239 and 10240 are separated by 242 nucleotides10240 and 10241 are separated by 213 nucleotides10241 and 10242 are separated by 151 nucleotides mRNA_1870: 10238 - K18043 OCA1 tyrosine-protein phosphatase OCA1, at 406,751 to 407,606 10238 mRNA_1871: 10239 - K14855 RSA4, NLE1 ribosome assembly protein 4, at 407,656 to 409,995 10239 mRNA_1872: 10240 - K14566 UTP24, FCF1 U3 small nucleolar RNA-associated protein 24, at 410,238 to 411,340 10240 mRNA_1873: 10241 - BLAST Rab geranylgeranyltransferase [Mycena chlorophos], at 411,554 to 414,002 10241 mRNA_1874: 10242 - BLAST midasin [Pseudozyma hubeiensis SY62], at 414,154 to 415,207 10242
Group Condition mRNA_1870 mRNA_1871 mRNA_1872 mRNA_1873 mRNA_1874
10238 10239 10240 10241 10242
ypd buoyancy 40 hrs No Buoyancy Separation -2.6 N.D. N.D. +0.2 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. N.D. -1.0 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 N.D. N.D. +0.2 -0.4
auxotrophy YNB Glucose -0.5 N.D. N.D. +0.5 -0.5
growth on oleic acid YNB Oleic Acid -0.4 N.D. N.D. +0.3 -0.5
auxotrophy Dropout Complete -0.6 N.D. N.D. +0.4 -0.4
growth on ypd YPD +0.1 N.D. N.D. -0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. N.D. +0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. +0.4 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. N.D. -0.3 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. -0.3 +0.5
growth on ricinoleic acid YNB Glucose +0.3 N.D. N.D. +0.4 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 N.D. N.D. -0.3 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. N.D. +0.1 +0.1
growth on oleic acid YNB Glucose +0.5 N.D. N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.2 N.D. N.D. -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. N.D. +0.5 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. N.D. +1.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. +1.3 +0.1
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