Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10245 and 10246 are separated by 294 nucleotides10246 and 10247 are separated by 364 nucleotides10247 and 10248 are separated by 141 nucleotides10248 and 10249 are separated by 583 nucleotides mRNA_1877: 10245 - K03363 CDC20 cell division cycle 20, cofactor of APC complex, at 418,587 to 420,557 10245 mRNA_1878: 10246 - K01875 SARS, serS seryl-tRNA synthetase, at 420,852 to 422,750 10246 mRNA_1879: 10247 - HMMPfam-MYND finger-PF01753,ProSiteProfiles-Zinc finger MYND-type profile.-PS50865,SUPERFAMILY--SSF144232, at 423,115 to 424,156 10247 mRNA_1880: 10248 - KOG0254 Predicted transporter (major facilitator superfamily), at 424,298 to 426,105 10248 mRNA_1881: 10249 - K11770 SFH1 chromatin structure-remodeling complex subunit SFH1, at 426,689 to 428,081 10249
Group Condition mRNA_1877 mRNA_1878 mRNA_1879 mRNA_1880 mRNA_1881
10245 10246 10247 10248 10249
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.0 -0.9 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 -0.8 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 -0.7 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 -0.3 N.D.
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.0 -0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.3 -0.4 N.D.
growth on oleic acid YNB Glucose N.D. N.D. +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.1 +0.2 N.D.
growth on ypd YPD N.D. N.D. -0.0 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 +0.3 N.D.
auxotrophy YNB Glucose N.D. N.D. -0.1 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.0 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.2 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.4 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.2 +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.0 +0.6 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.2 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.5 +0.3 N.D.
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