Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10248 and 10249 are separated by 583 nucleotides10249 and 10250 are separated by 679 nucleotides10250 and 10251 are separated by 323 nucleotides10251 and 10252 are separated by 615 nucleotides mRNA_1880: 10248 - KOG0254 Predicted transporter (major facilitator superfamily), at 424,298 to 426,105 10248 mRNA_1881: 10249 - K11770 SFH1 chromatin structure-remodeling complex subunit SFH1, at 426,689 to 428,081 10249 mRNA_1882: 10250 - HMMPfam-S4 domain-PF01479,ProSitePatterns-Ribosomal protein S4 signature.-PS00632,ProSiteProfiles-S4 RNA-binding domain profile.-PS50889,SMART-S4 RNA-binding domain-SM00363,SUPERFAMILY--SSF55174, at 428,761 to 429,844 10250 mRNA_1883: 10251 - KOG2665 Predicted FAD-dependent oxidoreductase, at 430,168 to 431,962 10251 mRNA_1884: 10252 - Hypothetical Protein, at 432,578 to 433,282 10252
Group Condition mRNA_1880 mRNA_1881 mRNA_1882 mRNA_1883 mRNA_1884
10248 10249 10250 10251 10252
auxotrophy Dropout Complete -0.0 N.D. N.D. -0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. N.D. +0.0 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 N.D. N.D. +0.2 -0.0
growth on ypd YPD +0.2 N.D. N.D. -0.1 -0.7
growth on ricinoleic acid YNB Glucose -0.4 N.D. N.D. -0.0 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.7 N.D. N.D. +0.0 +0.2
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. -0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. N.D. -0.1 -0.3
growth on oleic acid YNB Glucose -0.1 N.D. N.D. +0.0 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. N.D. +0.2 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. N.D. -0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. N.D. +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. N.D. -0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. +0.3 -0.0
auxotrophy YNB Glucose +0.3 N.D. N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.6 N.D. N.D. -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. N.D. +0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.6 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. N.D. +0.5 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. N.D. +0.3 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. N.D. +0.1 +0.4
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