Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10253 and 10254 are separated by 447 nucleotides10254 and 10255 are separated by 549 nucleotides10255 and 10256 are separated by 742 nucleotides10256 and 10257 are separated by 89 nucleotides mRNA_1885: 10253 - KOG1238 Glucose dehydrogenase/choline dehydrogenase/mandelonitrile lyase (GMC oxidoreductase family), at 433,544 to 436,141 10253 mRNA_1886: 10254 - KOG1470 Phosphatidylinositol transfer protein PDR16 and related proteins, at 436,589 to 438,730 10254 mRNA_1887: 10255 - K14823 EBP2, EBNA1BP2 rRNA-processing protein EBP2, at 439,280 to 440,428 10255 mRNA_1888: 10256 - HMMPfam-Protein of unknown function (DUF2423)-PF10338, at 441,171 to 441,971 10256 mRNA_1889: 10257 - Hypothetical Protein, at 442,061 to 442,321 10257
Group Condition mRNA_1885 mRNA_1886 mRNA_1887 mRNA_1888 mRNA_1889
10253 10254 10255 10256 10257
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 N.D. -1.9 -0.4
growth on ypd YPD -0.4 +0.2 N.D. -0.8 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.1 N.D. -0.9 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -0.4 N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.4 N.D. +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 N.D. +0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 N.D. +0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.0 N.D. +0.4 -0.1
growth on oleic acid YNB Oleic Acid -0.2 -0.1 N.D. +0.7 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.2 N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.1 N.D. +0.6 +0.2
auxotrophy Dropout Complete -0.1 +0.0 N.D. +1.0 -0.2
growth on oleic acid YNB Glucose -0.1 +0.1 N.D. +0.8 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 N.D. +0.7 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. +0.9 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.3 N.D. +0.7 +0.0
auxotrophy YNB Glucose -0.3 +0.5 N.D. +0.8 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.2 N.D. +1.4 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.3 N.D. +1.2 -0.0
growth on ricinoleic acid YNB Glucose -0.0 +0.1 N.D. +1.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.0 N.D. +1.7 +0.3
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