Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10259 and 10260 are separated by 596 nucleotides10260 and 10261 are separated by 653 nucleotides10261 and 10262 are separated by 361 nucleotides10262 and 10263 are separated by 115 nucleotides mRNA_1891: 10259 - K03083 GSK3B glycogen synthase kinase 3 beta, at 443,414 to 444,929 10259 mRNA_1892: 10260 - K15743 CES3_5 carboxylesterase 3/5, at 445,526 to 447,987 10260 mRNA_1893: 10261 - HMMPfam-Amidohydrolase-PF04909,SUPERFAMILY--SSF51556, at 448,641 to 450,220 10261 mRNA_1894: 10262 - K01253 EPHX1 microsomal epoxide hydrolase, at 450,582 to 452,258 10262 mRNA_1895: 10263 - K09247 CYP1, HAP1 heme activator protein 1, at 452,374 to 455,740 10263
Group Condition mRNA_1891 mRNA_1892 mRNA_1893 mRNA_1894 mRNA_1895
10259 10260 10261 10262 10263
facs on lipid accumulation Sorted High BODIPY Gate -3.3 -0.1 +0.1 -0.4 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid -3.1 +0.3 +0.3 +0.1 -0.1
growth on oleic acid YNB Oleic Acid -2.6 -0.3 +0.2 +0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -2.2 -0.3 +0.4 +0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.0 -0.0 +0.2 +0.1 -0.6
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.7 -0.2 -0.3 -0.5
growth on oleic acid YNB Glucose -0.3 -0.3 +0.3 +0.0 -0.7
auxotrophy YNB Glucose +0.1 -0.0 -0.2 -0.2 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.2 +0.4 +0.0 -0.6
ypd buoyancy 40 hrs Median Buoyancy -0.5 +0.3 -0.2 -0.3 +0.1
auxotrophy Dropout Complete +0.4 -0.3 -0.1 -0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.1 -0.1 +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.8 -0.3 +0.0 -0.2 -0.6
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 -0.0 -0.5 +0.1
growth on ricinoleic acid YNB Glucose +0.1 -0.1 +0.1 +0.1 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 -0.1 -0.0 -0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.2 -0.1 -0.2 +0.3
growth on ypd YPD +0.6 +0.1 +0.1 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.1 -0.2 -0.1 -0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 +0.5 -0.0 +0.4 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +2.2 -0.1 +0.0 +0.0 -0.5
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