Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10269 and 10270 are separated by 120 nucleotides10270 and 10271 are separated by 1764 nucleotides10271 and 10272 are separated by 745 nucleotides10272 and 10273 are separated by 205 nucleotides mRNA_1901: 10269 - HMMPfam-Spo7-like protein-PF03907, at 468,442 to 470,059 10269 mRNA_1902: 10270 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 470,180 to 472,538 10270 mRNA_1903: 10271 - HMMPfam-Sfi1 spindle body protein-PF08457, at 474,303 to 478,311 10271 mRNA_1904: 10272 - HMMPfam-Protein kinase domain-PF00069,ProSitePatterns-Serine/Threonine protein kinases active-site signature.-PS00108,ProSiteProfiles-Protein kinase domain profile.-PS50011,SMART-Serine/Threonine protein kinases, catalytic domain-SM00220,SUPERFAMILY--SSF56112, at 479,057 to 480,594 10272 mRNA_1905: 10273 - Hypothetical Protein, at 480,800 to 481,665 10273
Group Condition mRNA_1901 mRNA_1902 mRNA_1903 mRNA_1904 mRNA_1905
10269 10270 10271 10272 10273
facs on lipid accumulation Sorted Low BODIPY Gate -1.4 -0.9 N.D. -0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.9 +0.4 N.D. -0.5 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 -0.3 N.D. -0.4 -0.2
growth on ricinoleic acid YNB Glucose -0.6 +0.1 N.D. -0.4 -0.1
auxotrophy Dropout Complete -0.5 -0.0 N.D. -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.5 +0.2 N.D. -0.2 -0.1
auxotrophy YNB Glucose -0.4 -0.1 N.D. -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.3 N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.1 N.D. -0.4 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.2 N.D. -0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.6 +0.1 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.5 N.D. -0.1 -0.1
growth on oleic acid YNB Oleic Acid +0.0 +0.0 N.D. -0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.3 N.D. -0.1 +0.0
growth on ypd YPD +0.3 +0.1 N.D. -0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.9 -0.4 N.D. -0.3 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.1 N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 N.D. +0.1 -0.1
growth on oleic acid YNB Glucose +0.3 +0.3 N.D. +0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.1 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 +0.6 N.D. +0.1 +0.1
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