Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10273 and 10274 are separated by 1346 nucleotides10274 and 10275 are separated by 3747 nucleotides10275 and 10276 are separated by 1345 nucleotides10276 and 10277 are separated by 581 nucleotides mRNA_1905: 10273 - Hypothetical Protein, at 480,800 to 481,665 10273 mRNA_1906: 10274 - KOG0627 Heat shock transcription factor, at 483,012 to 484,134 10274 mRNA_1907: 10275 - Hypothetical Protein, at 487,882 to 489,189 10275 mRNA_1908: 10276 - K01426 E3.5.1.4, amiE amidase, at 490,535 to 492,598 10276 mRNA_1909: 10277 - K01426 E3.5.1.4, amiE amidase, at 493,180 to 495,383 10277
Group Condition mRNA_1905 mRNA_1906 mRNA_1907 mRNA_1908 mRNA_1909
10273 10274 10275 10276 10277
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -2.7 N.D. -0.3 -0.2
growth on oleic acid YNB Oleic Acid -0.3 -2.0 N.D. -0.6 -0.1
growth on oleic acid YNB Glucose -0.2 -2.0 N.D. -0.7 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -2.0 N.D. -0.5 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -2.6 N.D. +0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.0 -1.7 N.D. -0.3 -0.2
auxotrophy Dropout Complete -0.1 -1.6 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.1 -1.4 N.D. -0.0 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 -2.4 N.D. +0.0 +0.4
auxotrophy YNB Glucose -0.0 -1.3 N.D. -0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 -1.3 N.D. +0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.7 N.D. +0.1 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.0 -1.0 N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. -0.6 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.2 N.D. -0.5 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.6 N.D. +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 N.D. -0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.2 N.D. +0.1 +0.3
growth on ypd YPD +0.1 -0.5 N.D. +0.1 +0.6
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.3 N.D. +0.2 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.5 N.D. +0.5 +0.6
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