Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10280 and 10281 are separated by 578 nucleotides10281 and 10282 are separated by 309 nucleotides10282 and 10283 are separated by 552 nucleotides10283 and 10284 are separated by 78 nucleotides mRNA_1912: 10280 - K04450 ATF2, CREBP1 cyclic AMP-dependent transcription factor ATF-2, at 500,203 to 502,614 10280 mRNA_1913: 10281 - K04564 SOD2 superoxide dismutase, Fe-Mn family, at 503,193 to 504,426 10281 mRNA_1914: 10282 - K07942 ARL1 ADP-ribosylation factor-like protein 1, at 504,736 to 505,677 10282 mRNA_1915: 10283 - K03063 PSMC4, RPT3 26S proteasome regulatory subunit T3, at 506,230 to 507,925 10283 mRNA_1916: 10284 - HMMPfam-bZIP transcription factor-PF00170,HMMPfam-Domain of unknown function (DUF3425)-PF11905,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 508,004 to 509,890 10284
Group Condition mRNA_1912 mRNA_1913 mRNA_1914 mRNA_1915 mRNA_1916
10280 10281 10282 10283 10284
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. -0.3 N.D. -1.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. -1.4 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.4 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.8 N.D. -1.5
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.4 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.2 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. +0.5 N.D. +0.0
growth on ricinoleic acid YNB Glucose +0.4 N.D. +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.2 N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.1 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.0 N.D. +0.6
growth on oleic acid YNB Oleic Acid +0.5 N.D. +0.4 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 N.D. +0.8 N.D. -0.8
growth on ypd YPD +0.2 N.D. +0.4 N.D. +0.3
growth on oleic acid YNB Glucose +0.7 N.D. -0.0 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. +0.7 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.8 N.D. +0.5 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.5 N.D. +0.9
auxotrophy YNB Glucose +1.1 N.D. +0.7 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +1.2 N.D. +0.2 N.D. +0.8
auxotrophy Dropout Complete +0.9 N.D. +0.7 N.D. +0.5
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