Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10282 and 10283 are separated by 552 nucleotides10283 and 10284 are separated by 78 nucleotides10284 and 10285 are separated by 301 nucleotides10285 and 10286 are separated by 1384 nucleotides mRNA_1914: 10282 - K07942 ARL1 ADP-ribosylation factor-like protein 1, at 504,736 to 505,677 10282 mRNA_1915: 10283 - K03063 PSMC4, RPT3 26S proteasome regulatory subunit T3, at 506,230 to 507,925 10283 mRNA_1916: 10284 - HMMPfam-bZIP transcription factor-PF00170,HMMPfam-Domain of unknown function (DUF3425)-PF11905,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 508,004 to 509,890 10284 mRNA_1917: 10285 - K04488 iscU, nifU nitrogen fixation protein NifU and related proteins, at 510,192 to 511,246 10285 mRNA_1918: 10286 - BLAST protein of unknown function [Taphrina deformans PYCC ..., at 512,631 to 512,981 10286
Group Condition mRNA_1914 mRNA_1915 mRNA_1916 mRNA_1917 mRNA_1918
10282 10283 10284 10285 10286
facs on lipid accumulation Sorted Low BODIPY Gate -1.4 N.D. -0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -1.0 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.8 N.D. -1.5 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -0.2 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.0 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 N.D. -0.8 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.2 N.D. N.D.
growth on oleic acid YNB Glucose -0.0 N.D. +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. +0.6 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.4 N.D. -0.0 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.5 N.D. +0.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.6 N.D. N.D.
growth on ypd YPD +0.4 N.D. +0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. +0.4 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.8 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. +0.3 N.D. N.D.
auxotrophy YNB Glucose +0.7 N.D. +0.3 N.D. N.D.
auxotrophy Dropout Complete +0.7 N.D. +0.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. +0.9 N.D. N.D.
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