Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10285 and 10286 are separated by 1384 nucleotides10286 and 10287 are separated by 472 nucleotides10287 and 10288 are separated by 305 nucleotides10288 and 10289 are separated by 638 nucleotides mRNA_1917: 10285 - K04488 iscU, nifU nitrogen fixation protein NifU and related proteins, at 510,192 to 511,246 10285 mRNA_1918: 10286 - BLAST protein of unknown function [Taphrina deformans PYCC ..., at 512,631 to 512,981 10286 mRNA_1919: 10287 - K11340 ACTL6A, INO80K actin-like protein 6A, at 513,454 to 515,296 10287 mRNA_1920: 10288 - Hypothetical Protein, at 515,602 to 517,203 10288 mRNA_1921: 10289 - KOG4373 Predicted 3'-5' exonuclease, at 517,842 to 518,810 10289
Group Condition mRNA_1917 mRNA_1918 mRNA_1919 mRNA_1920 mRNA_1921
10285 10286 10287 10288 10289
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.4 -2.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.7 -2.2
auxotrophy Dropout Complete N.D. N.D. N.D. -0.3 -1.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.2 -1.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. +0.1 -1.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.7 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.3 -0.6
auxotrophy YNB Glucose N.D. N.D. N.D. -0.3 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.5 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.4 +0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.3 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.1 +0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.5 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.7 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.0 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.9 -0.1
growth on ypd YPD N.D. N.D. N.D. -0.4 +1.5
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