Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10293 and 10294 are separated by 221 nucleotides10294 and 10295 are separated by 572 nucleotides10295 and 10296 are separated by 1087 nucleotides10296 and 10297 are separated by 1100 nucleotides mRNA_1925: 10293 - K13239 PECI peroxisomal 3,2-trans-enoyl-CoA isomerase, at 523,662 to 525,143 10293 mRNA_1926: 10294 - HMMPfam-GDSL-like Lipase/Acylhydrolase family-PF13472,SUPERFAMILY--SSF52266, at 525,365 to 527,827 10294 mRNA_1927: 10295 - K19691 NIK1, TCSC osomolarity two-component system, sensor histidine kinase NIK1, at 528,400 to 533,209 10295 mRNA_1928: 10296 - Hypothetical Protein, at 534,297 to 534,878 10296 mRNA_1929: 10297 - K07880 RAB4B Ras-related protein Rab-4B, at 535,979 to 537,358 10297
Group Condition mRNA_1925 mRNA_1926 mRNA_1927 mRNA_1928 mRNA_1929
10293 10294 10295 10296 10297
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.1 -0.7 +0.1 -1.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 -0.7 +0.0 -0.3
growth on oleic acid YNB Glucose -0.4 -0.1 -0.3 -0.3 +0.3
growth on ypd YPD -0.7 +0.0 -0.1 +0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.4 -0.1 -0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 -0.3 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.2 -0.4 -0.0 +0.2
growth on oleic acid YNB Oleic Acid -0.3 -0.1 -0.1 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.1 -0.2 -0.4 +0.4
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 -0.4 -0.3 +0.6
auxotrophy Dropout Complete -0.1 +0.2 -0.3 -0.4 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.1 +0.2 -0.0 +0.3
growth on ricinoleic acid YNB Glucose -0.1 +0.3 -0.3 -0.1 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 +0.5 -0.1 -0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.7 -0.2 +0.3 -0.4
auxotrophy YNB Glucose +0.0 +0.5 -0.4 -0.5 +0.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.3 +0.2 -0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.4 -0.3 -0.4 +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.2 -0.1 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.4 -0.2 +0.3 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.6 +0.5 +0.3 -0.6
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