Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10295 and 10296 are separated by 1087 nucleotides10296 and 10297 are separated by 1100 nucleotides10297 and 10298 are separated by 346 nucleotides10298 and 10299 are separated by 1044 nucleotides mRNA_1927: 10295 - K19691 NIK1, TCSC osomolarity two-component system, sensor histidine kinase NIK1, at 528,400 to 533,209 10295 mRNA_1928: 10296 - Hypothetical Protein, at 534,297 to 534,878 10296 mRNA_1929: 10297 - K07880 RAB4B Ras-related protein Rab-4B, at 535,979 to 537,358 10297 mRNA_1930: 10298 - KOG1634 Predicted transcription factor DATF1, contains PHD and TFS2M domains, at 537,705 to 541,731 10298 mRNA_1931: 10299 - KOG2112 Lysophospholipase, at 542,776 to 544,439 10299
Group Condition mRNA_1927 mRNA_1928 mRNA_1929 mRNA_1930 mRNA_1931
10295 10296 10297 10298 10299
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 +0.1 -1.0 N.D. -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.7 +0.0 -0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.0 +0.2 N.D. -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.3 -0.4 N.D. -0.3
growth on ricinoleic acid YNB Glucose -0.3 -0.1 +0.4 N.D. -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 +0.4 N.D. -0.8
auxotrophy Dropout Complete -0.3 -0.4 +0.7 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.3 +0.1 N.D. -0.1
growth on oleic acid YNB Glucose -0.3 -0.3 +0.3 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.4 +0.4 N.D. -0.1
auxotrophy YNB Glucose -0.4 -0.5 +0.7 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 +0.0 N.D. +0.0
growth on ypd YPD -0.1 +0.2 -0.2 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.1 +0.3 N.D. -0.5
growth on oleic acid YNB Oleic Acid -0.1 -0.1 +0.3 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.3 +0.6 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.4 +0.9 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.3 +0.3 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.5 +0.3 -0.6 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 +0.3 N.D. +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.0 +0.3 N.D. +0.1
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