Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10296 and 10297 are separated by 1100 nucleotides10297 and 10298 are separated by 346 nucleotides10298 and 10299 are separated by 1044 nucleotides10299 and 10300 are separated by 287 nucleotides mRNA_1928: 10296 - Hypothetical Protein, at 534,297 to 534,878 10296 mRNA_1929: 10297 - K07880 RAB4B Ras-related protein Rab-4B, at 535,979 to 537,358 10297 mRNA_1930: 10298 - KOG1634 Predicted transcription factor DATF1, contains PHD and TFS2M domains, at 537,705 to 541,731 10298 mRNA_1931: 10299 - KOG2112 Lysophospholipase, at 542,776 to 544,439 10299 mRNA_1932: 10300 - BLAST family 69 glycosyltransferase [Melampsora larici-..., at 544,727 to 545,015 10300
Group Condition mRNA_1928 mRNA_1929 mRNA_1930 mRNA_1931 mRNA_1932
10296 10297 10298 10299 10300
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.0 N.D. -0.5 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.4 N.D. -0.8 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.3 N.D. -0.5 -0.7
growth on ricinoleic acid YNB Glucose -0.1 +0.4 N.D. -0.5 -0.6
growth on oleic acid YNB Oleic Acid -0.1 +0.3 N.D. -0.1 -0.5
growth on oleic acid YNB Glucose -0.3 +0.3 N.D. +0.0 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.4 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.3 N.D. -0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.3 N.D. +0.1 -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.6 N.D. +0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.4 N.D. -0.3 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.2 N.D. -0.6 +0.6
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.1 N.D. -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 N.D. +0.0 +0.4
auxotrophy Dropout Complete -0.4 +0.7 N.D. -0.5 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.3 N.D. +0.0 -0.0
auxotrophy YNB Glucose -0.5 +0.7 N.D. +0.0 +0.4
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.6 N.D. +0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.9 N.D. -0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.3 N.D. -0.1 +0.7
growth on ypd YPD +0.2 -0.2 N.D. -0.0 +3.7
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