Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10297 and 10298 are separated by 346 nucleotides10298 and 10299 are separated by 1044 nucleotides10299 and 10300 are separated by 287 nucleotides10300 and 10301 are separated by 435 nucleotides mRNA_1929: 10297 - K07880 RAB4B Ras-related protein Rab-4B, at 535,979 to 537,358 10297 mRNA_1930: 10298 - KOG1634 Predicted transcription factor DATF1, contains PHD and TFS2M domains, at 537,705 to 541,731 10298 mRNA_1931: 10299 - KOG2112 Lysophospholipase, at 542,776 to 544,439 10299 mRNA_1932: 10300 - BLAST family 69 glycosyltransferase [Melampsora larici-..., at 544,727 to 545,015 10300 mRNA_1933: 10301 - Hypothetical Protein, at 545,451 to 545,690 10301
Group Condition mRNA_1929 mRNA_1930 mRNA_1931 mRNA_1932 mRNA_1933
10297 10298 10299 10300 10301
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 N.D. -0.5 -0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +0.1 -0.6 -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. -0.8 -0.7 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.5 -0.7 +0.2
growth on ricinoleic acid YNB Glucose +0.4 N.D. -0.5 -0.6 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. -0.1 +0.3 -0.3
growth on oleic acid YNB Oleic Acid +0.3 N.D. -0.1 -0.5 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. +0.1 +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.3 +0.5 +0.1
growth on oleic acid YNB Glucose +0.3 N.D. +0.0 -0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.1 +0.3 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.0 +0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. -0.6 +0.6 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. -0.1 +0.7 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.0 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. -0.1 -0.1 +0.6
auxotrophy YNB Glucose 75mM Methionine +0.6 N.D. +0.2 +0.2 +0.4
auxotrophy Dropout Complete +0.7 N.D. -0.5 +0.7 +0.6
auxotrophy YNB Glucose 75mM Arginine +0.9 N.D. -0.1 +0.3 +0.5
auxotrophy YNB Glucose +0.7 N.D. +0.0 +0.4 +0.6
growth on ypd YPD -0.2 N.D. -0.0 +3.7 +0.2
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